Journal of Gastroenterology

, Volume 45, Issue 5, pp 478–487

Rikkunshito, a traditional Japanese medicine, may relieve abdominal symptoms in rats with experimental esophagitis by improving the barrier function of epithelial cells in esophageal mucosa

  • Hiroto Miwa
  • Junichi Koseki
  • Tadayuki Oshima
  • Takashi Kondo
  • Toshihiko Tomita
  • Jiro Watari
  • Takayuki Matsumoto
  • Tomohisa Hattori
  • Kunitsugu Kubota
  • Seiichi Iizuka
Original Article—Alimentary Tract

DOI: 10.1007/s00535-009-0180-1

Cite this article as:
Miwa, H., Koseki, J., Oshima, T. et al. J Gastroenterol (2010) 45: 478. doi:10.1007/s00535-009-0180-1

Abstract

Background

A traditional Japanese medicine, rikkunshito, has been reported to relieve dyspepsia symptoms. We investigated the effect of rikkunshito on RE-induced abdominal dyspepsia, and performed experiments to elucidate the mechanism of that effect.

Methods

RE model rats were prepared using 8-week-old male Wistar rats, and rikkunshito was administered in drinking water. Voluntary movement was used as an index of RE-induced abdominal dyspepsia, which was monitored by an infrared sensor. On the tenth day after surgery, the total area of esophageal erosion was measured, and samples of nonerosive mucosa were collected. Using those samples, intercellular spaces of epithelial mucosa were examined by transmission electron microscopy, and the NP-40-soluble and -insoluble levels of the tight junction proteins claudin-1, -3 and -4 and their mRNAs were determined.

Results

Rikkunshito did not reduce the average total area of erosive lesions in the esophageal mucosa of RE model rats. On day 10, voluntary movement was significantly decreased in the RE model rats and rikkunshito significantly increased it. Nonerosive esophageal mucosa from RE rats showed dilation of intercellular spaces in epithelium, and significantly decreased claudin-3 mRNA and protein levels. Rikkunshito significantly suppressed intercellular space dilation and significantly increased the level of NP-40-insoluble claudin-3, but it did not affect the mRNA level, suggesting that it promoted tight junction formation by facilitating the translocation of proteins.

Conclusion

Rikkunshito increased voluntary movement in RE model rats. This may have been because rikkunshito ameliorated the symptoms of RE by improving the barrier function of esophageal mucosa.

Keywords

GERDExperimental esophagitisTight junctionClaudinDilated intercellular spacesAnimal model

Copyright information

© Springer 2009

Authors and Affiliations

  • Hiroto Miwa
    • 1
  • Junichi Koseki
    • 1
    • 3
  • Tadayuki Oshima
    • 1
  • Takashi Kondo
    • 1
  • Toshihiko Tomita
    • 1
  • Jiro Watari
    • 1
  • Takayuki Matsumoto
    • 2
  • Tomohisa Hattori
    • 3
  • Kunitsugu Kubota
    • 3
  • Seiichi Iizuka
    • 3
  1. 1.Division of Upper Gastroenterology, Department of Internal MedicineHyogo College of MedicineNishinomiyaJapan
  2. 2.Division of Lower Gastroenterology, Department of Internal MedicineHyogo College of MedicineNishinomiyaJapan
  3. 3.Tsumura Research Laboratories, Tsumura & Co.IbarakiJapan