Journal of Gastroenterology

, Volume 44, Issue 5, pp 372–379

The role of hedgehog signaling during gastric regeneration

Authors

  • Dae-Hwan Kang
    • Department of Internal Medicine, School of MedicinePusan National University
  • Myoung-Eun Han
    • Department of Anatomy, School of MedicinePusan National University
    • Medical Research Center for Ischemic Tissue RegenerationPusan National University
  • Moo-Ho Song
    • Department of Anatomy, School of MedicinePusan National University
  • Young-Suk Lee
    • Department of Anatomy, School of MedicinePusan National University
  • Eun-Hee Kim
    • Department of Anatomy, School of MedicinePusan National University
  • Hyun-Jung Kim
    • Department of Anatomy, School of MedicinePusan National University
  • Gwang-Ha Kim
    • Department of Internal Medicine, School of MedicinePusan National University
  • Dong-Heon Kim
    • Department of Surgery, School of MedicinePusan National University
  • Sik Yoon
    • Department of Anatomy, School of MedicinePusan National University
    • Medical Research Center for Ischemic Tissue RegenerationPusan National University
  • Sun-Yong Baek
    • Department of Anatomy, School of MedicinePusan National University
  • Bong-Seon Kim
    • Department of Anatomy, School of MedicinePusan National University
  • Jae-Bong Kim
    • Department of Anatomy, School of MedicinePusan National University
    • Department of Anatomy, School of MedicinePusan National University
    • Medical Research Center for Ischemic Tissue RegenerationPusan National University
Original Article—Alimentary Tract

DOI: 10.1007/s00535-009-0006-1

Cite this article as:
Kang, D., Han, M., Song, M. et al. J Gastroenterol (2009) 44: 372. doi:10.1007/s00535-009-0006-1

Abstract

Background

Hedgehog signaling plays critical roles during embryonic development. It is also involved in tissue regeneration and carcinogenesis in various adult tissues. Moreover, it regulates the maintenance of cancer stem cells and adult stem cells. Although hedgehog signaling is important in gastric carcinogenesis, its role in gastric regeneration has not been previously examined. In the present study, we evaluated the expression and roles of hedgehog signaling during gastric regeneration.

Methods

Gastric ulcers were induced by serosal application of an acetic acid solution in mice. Sham-operated mice served as controls. The proliferation of gastric progenitor cells was studied using bromodeoxyuridine (BrdU). The expression of hedgehog signaling molecules and the differentiation of gastric progenitor cells were examined by immunohistochemical staining and Western blotting.

Results

One day after the induction of gastric ulcer, the proliferation of gastric progenitor cells increased; however, the expression of hedgehog signaling molecules, including sonic hedgehog (Shh), Indian hedgehog (Ihh), desert hedgehog (Dhh), and patched (Ptch1) decreased at the ulcer margin. From 5 days after the induction of gastric ulcer, newly generated gastric glands and their differentiation were observed at the ulcer margin. The expression of hedgehog signaling molecules gradually increased in the newly generated gastric glands of the ulcer margin. Cyclopamine, a specific inhibitor of hedgehog signaling, significantly inhibited the differentiation of mucous cells and parietal cells during the gastric regeneration process.

Conclusion

The above results suggest that hedgehog signaling is involved in the differentiation of gastric progenitor cells during the gastric ulcer repair process.

Keywords

Hedgehog signaling Gastric regeneration Differentiation Parietal cells Mucous cells

Supplementary material

535_2009_6_MOESM1_ESM.jpg (2.8 mb)
Supplementary Fig.1 (JPEG 2901 kb)

Copyright information

© Springer 2009