Journal of Hepato-Biliary-Pancreatic Surgery

, Volume 16, Issue 4, pp 513–520

Tissue factor expression demonstrates severe sinusoidal endothelial cell damage during rejection after living-donor liver transplantation

Authors

    • Department of Hepatobiliary Pancreatic and Transplant Surgery, Graduate School of MedicineMie University
  • Naohisa Kuriyama
    • Department of Hepatobiliary Pancreatic and Transplant Surgery, Graduate School of MedicineMie University
  • Masashi Kisawada
    • Department of Hepatobiliary Pancreatic and Transplant Surgery, Graduate School of MedicineMie University
  • Takashi Hamada
    • Department of Hepatobiliary Pancreatic and Transplant Surgery, Graduate School of MedicineMie University
  • Shugo Mizuno
    • Department of Hepatobiliary Pancreatic and Transplant Surgery, Graduate School of MedicineMie University
  • Hiroyuki Sakurai
    • Department of Hepatobiliary Pancreatic and Transplant Surgery, Graduate School of MedicineMie University
  • Masami Tabata
    • Department of Hepatobiliary Pancreatic and Transplant Surgery, Graduate School of MedicineMie University
  • Hiroshi Imai
    • Department of Pathology, Graduate School of MedicineMie University
  • Kohji Okamoto
    • First Department of SurgeryUniversity of Occupational and Environmental Health
  • Shinji Uemoto
    • Department of Hepatobiliary Pancreatic and Transplantation Surgery, Graduate School of MedicineKyoto University
  • Shuji Isaji
    • Department of Hepatobiliary Pancreatic and Transplant Surgery, Graduate School of MedicineMie University
Original article

DOI: 10.1007/s00534-009-0095-5

Cite this article as:
Usui, M., Kuriyama, N., Kisawada, M. et al. J Hepatobiliary Pancreat Surg (2009) 16: 513. doi:10.1007/s00534-009-0095-5

Abstract

Background/Purpose

Since it is well known that endothelial cells may be important targets during rejection after living-donor liver transplantation, in this study we investigated liver sinusoidal endothelial cell (SEC) damage during rejection by focusing on thrombomodulin (TM) and hyaluronic acid (HA) as plasma markers of SEC damage. We also examined tissue factor (TF) expression in SECs, because damage to endothelial cells leads to immediate activation of the coagulation system, with the damage being triggered mainly by TF.

Methods

Living-donor liver transplantation was performed at Mie University Hospital between March 2002 and December 2007; there were 8 patients with rejection (4 with acute cellular rejection and 4 with chronic rejection) and 32 patients without rejection. Liver biopsy tissue was immunostained with an anti-TF antibody, and assessed for SEC damage. In addition, total RNA was extracted from liver biopsy tissue and tested for TF mRNA expression by reverse-transcription polymerase chain reaction (RT-PCR).

Results

The plasma TM level was significantly higher in the rejection group than in the non-rejection group. TF expression was observed in SECs, in infiltrating inflammatory cells, and in the vascular endothelium in the rejection group. TF mRNA expression was significantly higher in the rejection group than in the non-rejection group.

Conclusions

We demonstrated that TF expression revealed severe SEC damage in grafted liver during both acute cellular rejection and chronic rejection.

Keywords

Tissue factorSinusoidal endothelial cellLiving-donor liver transplantationThrombomodulinHyaluronic acid

Copyright information

© Springer 2009