Choledocho-choledochostomy: the natural history of healing in pigs
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Aiming to investigate the natural history of the healing of choledocho-choledochostomies.
Fifty-five female pigs of 57 kg median weight were used for the experiments. The gallbladder was removed and the common bile duct transected. Continuity was re-established by standardized single-line, interrupted, and inverted sutures. The pigs had a planned postoperative survival of up to 14 days with a subsequent laparotomy for evaluation. Blood samples were drawn prior to the first and the final operations. During laparotomy the animals were investigated for signs of cholascos, and an intraoperative cholangiography was performed. The excised anastomosis was examined for breaking strength and collagen content.
Standard liver parameters were not significantly affected by the surgery, and cholangiography showed no signs of extrahepatic stenosis or intrahepatic dilatation. Breaking strength showed a decrease for the initial 3 postoperative days (PODs), then an increase to a stable level on PODs 6 to 14. Collagen content per volume showed a rise on PODs 0 to 1, then no change until POD 4, followed by a gradual rise until day 6. Subsequently a stable level was reached until POD 14. Two pigs were excluded due to minor cholascos.
The present study on pigs shows that choledocho-choledochostomies, judged by breaking strength and collagen content, regain a stable level of strength 6 days after operation.
- Northover, JM, Terblanche, J (1979) A new look at the arterial supply of the bile duct in man and its surgical implications. Br J Surg 66: pp. 379-84 CrossRef
- Watters, DA, Smith, AN, Eastwood, MA, Anderson, KC, Elton, RA, Mugerwa, JW (1985) Mechanical properties of the colon: comparison of the features of the African and European colon in vitro. Gut 26: pp. 384-92 CrossRef
- Woessner, JF Determination of hydroxyproline in connective tissue. In: Hall, DA eds. (1976) The methodology of connective tissue research. Joynson-Bruvvers, Oxford, pp. 227-33
- Mortensen, FV, Ishibashi, T, Hojo, N, Yasuda, Y (2004) A gallbladder flap for reconstruction of the common bile duct. an experimental study on pigs. J Hepatobiliary Pancreat Surg 11: pp. 112-5 CrossRef
- Christensen, M, Laursen, HB, Rokkjaer, M, Jensen, PF, Yasuda, Y, Mortensen, FV (2005) Reconstruction of the common bile duct by a vascular prosthetic graft: an experimental study in pigs. J Hepatobiliary Pancreat Surg 12: pp. 231-4 CrossRef
- Laursen, HB, Thorsoe, HJ, Funch-Jensen, P, Rokkjaer, M, Yasuda, Y, Mortensen, FV (2005) Robotic-Assisted laparoscopic Roux-en-Y choledochojejunostomy in pigs. J Hepatobiliary Pancreat Surg 12: pp. 167-72 CrossRef
- Northover, JM, Hickman, R, Watson, RG, Terblanche, J (1985) Healing of the bile duct anastomosis after transverse choledochotomy or transplantation of the liver in the pig. Surg Gynecol Obstet 160: pp. 33-6
- Christensen, H, Chemnitz, J, Christensen, BC, Oxlund, H (1995) Collagen structural organization of healing colonic anastomoses and the effect of growth hormone treatment. Dis Colon Rectum 38: pp. 1200-5 CrossRef
- Nelson, PG (1967) A tensiometer for the determination of wound strength. Aust N Z J Surg 37: pp. 75-7
- Choledocho-choledochostomy: the natural history of healing in pigs
Journal of Hepato-Biliary-Pancreatic Surgery
Volume 14, Issue 5 , pp 498-502
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- A1. Department of Gastrointestinal Surgery L, Aarhus University Hospital, NBG, 8000, Aarhus C, Denmark
- A2. Department of Connective Tissue Biology, The Institute of Anatomy, University of Aarhus, Denmark
- A3. Jichi Medical School, Tochigi, Japan