Sexual Plant Reproduction

, Volume 14, Issue 3, pp 135–142

Phenotypic complementation of ms45 maize requires tapetal expression of MS45

  • A. M. Cigan
  • E. Unger
  • R.-j. Xu
  • T. Kendall
  • T. W. Fox
Original Article

DOI: 10.1007/s004970100099

Cite this article as:
Cigan, A., Unger, E., Xu, R. et al. Sex Plant Reprod (2001) 14: 135. doi:10.1007/s004970100099

Abstract  

A mutation in the maize Ms45 gene results in a male-sterile phenotype due to the absence of pollen. Cytological examination of microspores in ms45 mutant plants reveals that the cell wall of the developing male gametophyte is poorly formed, while genetic analysis indicates that mutations at Ms45 are sporophytic. The Ms45 gene has been isolated by transposon tagging and transcription was shown by RNA hybridization analysis to be tassel-specific. In this report, an efficient system for molecular complementation is described and used to confirm that a transformed copy of the wild-type gene was able to fully restore fertility to ms45 mutant maize and to characterize MS45 protein expression in maize anthers. The MS45 protein was localized to the tapetum and expressed maximally during the early-vacuolate microspore stage of development. Based on these observations, phenotypic complementation of ms45 was used as an assay to determine tapetal cell-layer activity of other anther-specific and constitutive promoters.

Keywords MaizeMale fertilityMs45TapetumTransformation

Copyright information

© Springer-Verlag Berlin Heidelberg 2001

Authors and Affiliations

  • A. M. Cigan
    • 1
  • E. Unger
    • 1
  • R.-j. Xu
    • 1
  • T. Kendall
    • 1
  • T. W. Fox
    • 1
  1. 1.Agronomic Traits Department, Pioneer Hi-Bred International, 7300 NW 62nd Ave., Johnston, IA 50131 USA e-mail: Erica.Unger-Wallace@Pioneer.com Tel.: +1-515-270-3437, Fax: +1-515-253-2149US