Sexual Plant Reproduction

, Volume 13, Issue 5, pp 279–283

Flow cytometric evidence for endopolyploidization in cabbage (Brassica oleracea L.) flowers

  • N. Kudo
  • Yasuo Kimura
Short Communication

DOI: 10.1007/s004970100066

Cite this article as:
Kudo, N. & Kimura, Y. Sex Plant Reprod (2001) 13: 279. doi:10.1007/s004970100066


Flow cytometric analysis of the nuclear DNA contents of somatic tissues from flowers of cabbage (Brassica oleracea L.) revealed extensive endoreduplication, resulting in tissues that contain cells with multiple ploidy levels (also called ’endopolyploidy’). Multiples of the haploid nuclear genome complement (1C) corresponding to 2C, 4C, 8C, 16C, 32C and 64C were observed in mature flowers. The distribution of cells with the differerent ploidy levels is tissue-specific and is characteristic of the stage of development. Nuclei of young flower buds exclusively gave 2C and 4C peaks, indicating that the tissues maintained diploid level. Endoreduplication was consistently detected during flower development. Endopolyploidy is probably common in differentiation of cabbage plants. Implications of this original feature are discussed.

Keywords  Brassica oleraceaEndopolyploidyEndoreduplicationFlow cytometryFlower development

Copyright information

© Springer-Verlag Berlin Heidelberg 2001

Authors and Affiliations

  • N. Kudo
    • 1
  • Yasuo Kimura
    • 1
  1. 1.Division of Plant Biotechnology, Gunma Horticultural Experiment Station, 493 Nishi-Obokata, Sawa-Azuma, Gunma 379–2224, Japan, e-mail:, Tel.: +81-210-62-1021, Fax: +81-210-20-8016JP