Sexual Plant Reproduction

, Volume 13, Issue 5, pp 285–288

Isolation and characterization of OsDMC1, the rice homologue of the yeast DMC1 gene essential for meiosis

  • Zhao-jun Ding
  • T. Wang
  • Kang Chong
  • Shunong Bai
Sequence Update

DOI: 10.1007/s004970100065

Cite this article as:
Ding, Z., Wang, T., Chong, K. et al. Sex Plant Reprod (2001) 13: 285. doi:10.1007/s004970100065

Abstract 

Yeast DMC1 is a meiosis-specific gene required for homologous chromosome pairing in meiosis. Using degenerate primers designed according to amino acid motifs conserved in yeast Dmc1 and Arabidopsis AtDmc1, we obtained full-length cDNA of a rice homologue of the DMC1 gene (OsDMC1) by RT-PCR and rapid amplification of cDNA ends (RACEs). OsDmc1 exhibited 53% amino acid sequence identity to yeast Dmc1 and 81% to AtDmc1. OsDMC1 was expressed at high-levels in reproductive organs, low-levels in roots, and undetectable levels in leaves and seedlings. Southern blot analyses revealed that OsDMC1 is one of two DMC1 homologues present in rice.

Keywords MeiosisRiceDMC1

Copyright information

© Springer-Verlag Berlin Heidelberg 2001

Authors and Affiliations

  • Zhao-jun Ding
    • 1
  • T. Wang
    • 1
  • Kang Chong
    • 1
  • Shunong Bai
    • 2
  1. 1.Research Center for Plant Molecular and Developmental Biology, Institute of Botany, The Chinese Academy of Sciences, 20 Nanxincum, Xiangshan, Beijing 100093, China e-mail: twang@ns.ibcas.ac.cn Fax: +86-10-82594821CN
  2. 2.Department of Developmental Biology, Peking University, Beijing 100871, ChinaCN