Sexual Plant Reproduction

, Volume 13, Issue 5, pp 285–288

Isolation and characterization of OsDMC1, the rice homologue of the yeast DMC1 gene essential for meiosis

Authors

  • Zhao-jun Ding
    • Research Center for Plant Molecular and Developmental Biology, Institute of Botany, The Chinese Academy of Sciences, 20 Nanxincum, Xiangshan, Beijing 100093, China e-mail: twang@ns.ibcas.ac.cn Fax: +86-10-82594821
  • T. Wang
    • Research Center for Plant Molecular and Developmental Biology, Institute of Botany, The Chinese Academy of Sciences, 20 Nanxincum, Xiangshan, Beijing 100093, China e-mail: twang@ns.ibcas.ac.cn Fax: +86-10-82594821
  • Kang Chong
    • Research Center for Plant Molecular and Developmental Biology, Institute of Botany, The Chinese Academy of Sciences, 20 Nanxincum, Xiangshan, Beijing 100093, China e-mail: twang@ns.ibcas.ac.cn Fax: +86-10-82594821
  • Shunong Bai
    • Department of Developmental Biology, Peking University, Beijing 100871, China
Sequence Update

DOI: 10.1007/s004970100065

Cite this article as:
Ding, Z., Wang, T., Chong, K. et al. Sex Plant Reprod (2001) 13: 285. doi:10.1007/s004970100065
  • 196 Views

Abstract 

Yeast DMC1 is a meiosis-specific gene required for homologous chromosome pairing in meiosis. Using degenerate primers designed according to amino acid motifs conserved in yeast Dmc1 and Arabidopsis AtDmc1, we obtained full-length cDNA of a rice homologue of the DMC1 gene (OsDMC1) by RT-PCR and rapid amplification of cDNA ends (RACEs). OsDmc1 exhibited 53% amino acid sequence identity to yeast Dmc1 and 81% to AtDmc1. OsDMC1 was expressed at high-levels in reproductive organs, low-levels in roots, and undetectable levels in leaves and seedlings. Southern blot analyses revealed that OsDMC1 is one of two DMC1 homologues present in rice.

Keywords MeiosisRiceDMC1
Download to read the full article text

Copyright information

© Springer-Verlag Berlin Heidelberg 2001