Using S-locus deletions to evaluate self-incompatibility candidate genes: an example for a novel anther-expressed gene
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- Casselman, A., Nasrallah, M. & Nasrallah, J. Sex Plant Reprod (2000) 12: 227. doi:10.1007/s004970050005
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A novel gene, designated SPA, was isolated using a differential display strategy designed to target the Brassica self-incompatibility (S) locus for anther-expressed sequences. SPA is genetically and physically linked to the S locus and produces two transcripts expressed predominantly in anthers. SPA alleles exhibit a degree of sequence polymorphism comparable to that characteristic of the S locus glycoprotein and S receptor kinase genes which are known to be required for self-incompatibility in the stigma. Thus, SPA fulfills several of the criteria predicted for a gene that encodes the pollen component of self-incompatibility. However, analysis of an S-locus deletion that encompasses this gene rules out a role for this gene as a determinant of specificity in the self-incompatibility response.