Sexual Plant Reproduction

, Volume 12, Issue 4, pp 227–231

Using S-locus deletions to evaluate self-incompatibility candidate genes: an example for a novel anther-expressed gene

  • A. L. Casselman
  • M. E. Nasrallah
  • J. B. Nasrallah
Original Paper

DOI: 10.1007/s004970050005

Cite this article as:
Casselman, A., Nasrallah, M. & Nasrallah, J. Sex Plant Reprod (2000) 12: 227. doi:10.1007/s004970050005

Abstract 

A novel gene, designated SPA, was isolated using a differential display strategy designed to target the Brassica self-incompatibility (S) locus for anther-expressed sequences. SPA is genetically and physically linked to the S locus and produces two transcripts expressed predominantly in anthers. SPA alleles exhibit a degree of sequence polymorphism comparable to that characteristic of the S locus glycoprotein and S receptor kinase genes which are known to be required for self-incompatibility in the stigma. Thus, SPA fulfills several of the criteria predicted for a gene that encodes the pollen component of self-incompatibility. However, analysis of an S-locus deletion that encompasses this gene rules out a role for this gene as a determinant of specificity in the self-incompatibility response.

Key words Anther genesBrassicaS-locus deletionsSelf-incompatibility

Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • A. L. Casselman
    • 1
  • M. E. Nasrallah
    • 1
  • J. B. Nasrallah
    • 1
  1. 1.Department of Plant Biology, Cornell University, Ithaca, NY 14853, USA e-mail: jbn2@cornell.edu Tel.: +1-607-255-8189, Fax: +1-607-255-5407US