Sexual Plant Reproduction

, Volume 13, Issue 4, pp 177–183

Female meiosis in wild-type Arabidopsisthaliana and in two meiotic mutants

  • S. J. Armstrong
  • G. H. Jones
Original Article

DOI: 10.1007/s004970000050

Cite this article as:
Armstrong, S. & Jones, G. Sex Plant Reprod (2001) 13: 177. doi:10.1007/s004970000050

Abstract 

Female meiosis in Arabidopsis has been analysed cytogenetically using an adaptation of a technique previously applied to male meiosis. Meiotic progression was closely correlated with stages of floral development, including the length and morphology of the gynoecium. Meiosis in embryo sac mother cells (EMCs) occurs later in development than male meiosis, in gynoecia that range in size between 0.3 and 0.8 mm. The earliest stages in EMCs coincide with the second division to tetrad stages in pollen mother cells. However, the details of meiotic chromosome behaviour in EMCs correspond closely to the observations we have previously made in male meiosis. In addition, BrdU labelling coupled with an immunolocalisation detection system was used to mark the S phase in cells preceding their entry into prophase I. These techniques allow female meiotic stages of Arabidopsis to be analysed in detail, from the S-phase through to the tetrad stage, and are shown to be equally applicable to the analysis of female meiosis in meiotic mutants.

Keywords ArabidopsisMeiosisEmbryo sac mother cellsMegasporogenesis

Copyright information

© Springer-Verlag Berlin Heidelberg 2001

Authors and Affiliations

  • S. J. Armstrong
    • 1
  • G. H. Jones
    • 1
  1. 1.School of Biosciences, The University of Birmingham, B15 2TT, Birmingham, UK e-mail: s.j.armstrong@bham.ac.ukGB