Trees

, Volume 11, Issue 1, pp 59–64

Axenic mycorrhization of wild type and transgenic hybrid aspen expressing T-DNA indoleacetic acid-biosynthetic genes

  • R. Hampp
  • M. Ecke
  • C. Schaeffer
  • T. Wallenda
  • A. Wingler
  • I. Kottke
  • B. Sundberg
ORIGINAL ARTICLE

DOI: 10.1007/s004680050059

Cite this article as:
Hampp, R., Ecke, M., Schaeffer, C. et al. Trees (1996) 11: 59. doi:10.1007/s004680050059

Abstract

 We describe and document the in vitro synthesis of ectomycorrhiza between roots of wild type and transgenic aspen (Populus tremula × P. tremuloides), expressing Agrobacterium tumefaciens T-DNA indoleacetic acid (IAA)-biosynthetic genes, and Amanita muscaria. Plantlets were raised from tissue culture. The root system of approximately 4-week-old plantlets was transferred to Petri dishes and incubated together with fungal mycelia under sterile conditions. Ectomycorrhiza showing both a well developed hyphal mantle and Hartig net were established within 3 to 4 weeks. Formation and morphology of ectomycorrhiza were not affected by the transformation of aspen, expressing the IAA biosynthetic genes in roots. As both hybrid aspen and fungal cells can be genetically engineered, this system offers a new approach to the study of mycorrhizal symbioses.

Key words Amanita muscaria Ectomycorrhiza Hybrid aspen In vitro culture Transgenic IAA-overexpressing hybrid aspen 

Copyright information

© Springer-Verlag Berlin Heidelberg 1996

Authors and Affiliations

  • R. Hampp
    • 1
  • M. Ecke
    • 1
  • C. Schaeffer
    • 1
  • T. Wallenda
    • 1
  • A. Wingler
    • 2
  • I. Kottke
    • 3
  • B. Sundberg
    • 4
  1. 1.Physiologische Ökologie der Pflanzen, Universität Tübingen, Auf der Morgenstelle 1, D-72076 Tübingen, GermanyDE
  2. 2.Department of Animal and Plant Sciences, University of Sheffield, Sheffield S10 2TN, UKGB
  3. 3.Spezielle Botanik und Mykologie, Universität Tübingen, Auf der Morgenstelle 1, D-72076 Tübingen, GermanyDE
  4. 4.Department of Forest Genetics and Plant Physiology, The Swedish University of Agricultural Sciences, S-90183 Umeå, SwedenSE

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