Trees

, Volume 14, Issue 1, pp 10–16

The impact of ozone fumigation and fertilization on chlorophyll fluorescence of birch leaves (Betula pendula)

  • Sergej Shavnin
  • Stefan Maurer
  • Rainer Matyssek
  • Wolfgang Bilger
  • C. Scheidegger
ORIGINAL ARTICLE

DOI: 10.1007/s004680050002

Cite this article as:
Shavnin, S., Maurer, S., Matyssek, R. et al. Trees (1999) 14: 10. doi:10.1007/s004680050002

Abstract

 The impact of ozone fumigation on chlorophyll a fluorescence parameters and chlorophyll content of birch trees grown at high and low fertilization were studied for 6-, 8-, and 12-week old leaves. Fluorescence parameters were measured with a portable fluorometer with its fibre optics tightly inserted in a gas exchange cuvette at light intensities from 0 to 220 µmol photons m−2 s−1. Ozone caused significant changes of primary photosynthetic reactions: a decrease of the quantum yield of photosystem II and an increase of non-photochemical quenching. In all leaves a biphasic light response of non-photochemical quenching was observed. Ozone fumigation shifted the onset of the second phase from a PFD of about 60 µmol m−2 s−1 to about 30 µmol m−2 s−1. While the fertilizer concentration had no influence on this character, high fertilization supply of plants partially reduced O3-induced damage. The light responses of Ft, Fm′ and NPQ observed in birch leaves grown in O3-free air indicate the existence of at least two different processes governing energy conversion of the photosynthetic apparatus at PS II in the range of PFD 0–200 µmol photons m−2 s−1. The first phase was attributed to a rather slowly relaxing type of non-photochemical quenching, which, at least at low PFD, is thought to be related to a state 1–2 transition. The further changes of the fluorescence parameters studied at higher PFD might be explained by an increase of energy-dependent quenching, connected with the energization of the thylakoid membrane and zeaxanthin synthesis. A major effect of ozone treatment was a lowering of PS II quantum yield. This reflects a reduction of PS II electron transport and corresponds to the reduction of CO2-fixation observed in ozonated leaves.

Key words Ozone fumigationChlorophyll fluorescenceFertilizationLight intensitySenescence

Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • Sergej Shavnin
    • 1
  • Stefan Maurer
    • 2
  • Rainer Matyssek
    • 3
  • Wolfgang Bilger
    • 4
  • C. Scheidegger
    • 5
  1. 1.Dept. of Biophysics, Ural State Forestry Engineering Academy, Siberian trakt 37, 620032 Ekaterinburg, RussiaRU
  2. 2.Paul Scherrer Institute, CH-5232 Villigen PSI, SwitzerlandCH
  3. 3.Dept. of Forest Botany, Ludwig Maximilians-University Munich, Am Hochanger 13, D-85354 Freising, GermanyDE
  4. 4.Dept. of Biology, Agricultural Univ. P.O. Box 5014, N-1432 Å, NorwayNO
  5. 5.Swiss Federal Institute for Forest, Snow and Landscape Research, Zürcherstr. 111, CH-8903 Birmensdorf, Switzerland e-mail: scheidegger@wsl.ch Fax: 0049 1 739 2215; Tel.: 0049 1 739 2439CH