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Impact of abscisic acid in overcoming the problem of albinism in horse chestnut androgenic embryos

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Abstract

Horse chestnut (Aesculus hyppocastanum L., Hyppocastanacea) is a relict species with a slow and complex reproductive cycle considered to have horticultural and medical importance. The cycle maybe circumvented via in vitro androgenesis. Androgenesis of horse chestnut was induced in microspores and anther culture on MS media. Some of the horse chestnut androgenic embryos were albinos. Addition of abscisic acid in media (in concentrations of 0.01, 0.1, 0.5, 1, 2, 5, 10, and 20 mg l−1) with horse chestnut androgenic embryos has circumvented the reproduction cycle barriers. The best results were achieved on medium with the lowest abscisic acid concentration (0.01 mg l−1) in microspore culture. The microspore culture proved to be a better model system for embryo production and albino embryo reduction than anther culture. Flow cytometry analysis after maturation treatments induced by ABA showed that 88 % of green embryos originating from microspore culture were haploid. However, 50 % of green embryos from anther culture were haploid. The remaining analyzed androgenic embryos, from both types of cultures were diploid.

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Acknowledgments

This work was supported by the Ministry of Education and Science of Serbia, grant N0. 173015.

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Authors and Affiliations

  1. Institute for Biological Research “Siniša Stanković”, University of Belgrade, Despot Stefan Blvd 142, 11 000, Belgrade, Serbia

    Dušica Ćalić, Nina Devrnja, Jelena Milojević, Ljiljana Tubić & Snežana Zdravković-Korać

  2. Faculty of Biotechnology, University of Ljubljana, Jamnikarjeva 101, 1000, Ljubljana, Slovenia

    Borut Bohanec

  3. Institute for Multidisciplinary Research, University of Belgrade, Kneza Višeslava 1, 11 000, Belgrade, Serbia

    Igor Kostić

Authors
  1. Dušica Ćalić
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  2. Borut Bohanec
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  3. Nina Devrnja
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  4. Jelena Milojević
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  5. Ljiljana Tubić
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  6. Igor Kostić
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  7. Snežana Zdravković-Korać
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Correspondence to Dušica Ćalić.

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Communicated by D. Treutter.

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Ćalić, D., Bohanec, B., Devrnja, N. et al. Impact of abscisic acid in overcoming the problem of albinism in horse chestnut androgenic embryos. Trees 27, 755–762 (2013). https://doi.org/10.1007/s00468-012-0830-4

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  • DOI: https://doi.org/10.1007/s00468-012-0830-4

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