, Volume 26, Issue 9, pp 1483-1489
Date: 15 Mar 2011

Two-photon in vivo imaging of cells

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access


In vivo imaging of cells gives a glimpse into the world of biology in a natural setting unparalleled by any other venue. Two-photon imaging of fluorescently labeled cells has become the standard to obtain high-resolution, dynamic images of living specimens with great specificity. This review focuses on providing the reader with a short history of, and impetus behind, two-photon imaging, its working mechanics, and emerging technologies related to biological multiphoton imaging.