Two-photon in vivo imaging of cells
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- Christensen, D.J. & Nedergaard, M. Pediatr Nephrol (2011) 26: 1483. doi:10.1007/s00467-011-1818-9
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In vivo imaging of cells gives a glimpse into the world of biology in a natural setting unparalleled by any other venue. Two-photon imaging of fluorescently labeled cells has become the standard to obtain high-resolution, dynamic images of living specimens with great specificity. This review focuses on providing the reader with a short history of, and impetus behind, two-photon imaging, its working mechanics, and emerging technologies related to biological multiphoton imaging.