Pediatric Nephrology

, Volume 26, Issue 9, pp 1483–1489

Two-photon in vivo imaging of cells

Review

DOI: 10.1007/s00467-011-1818-9

Cite this article as:
Christensen, D.J. & Nedergaard, M. Pediatr Nephrol (2011) 26: 1483. doi:10.1007/s00467-011-1818-9

Abstract

In vivo imaging of cells gives a glimpse into the world of biology in a natural setting unparalleled by any other venue. Two-photon imaging of fluorescently labeled cells has become the standard to obtain high-resolution, dynamic images of living specimens with great specificity. This review focuses on providing the reader with a short history of, and impetus behind, two-photon imaging, its working mechanics, and emerging technologies related to biological multiphoton imaging.

Keywords

In vivoFluorescence imagingTwo-photon imagingLaser scanning microscopy

Copyright information

© IPNA 2011

Authors and Affiliations

  1. 1.The Institute of OpticsUniversity of RochesterRochesterUSA
  2. 2.Center for Translational NeuromedicineUniversity of Rochester Medical CenterRochesterUSA