Original Article

Pediatric Nephrology

, Volume 23, Issue 3, pp 395-401

First online:

Expression of mRNA for functional molecules in urinary sediment in glomerulonephritis

  • Koji TsugawaAffiliated withDepartment of Pediatrics, Hirosaki University School of Medicine Email author 
  • , Eishin OkiAffiliated withDepartment of Pediatrics, Hirosaki University School of Medicine
  • , Koichi SuzukiAffiliated withDepartment of Pediatrics, Hirosaki University School of Medicine
  • , Tadaatsu ImaizumiAffiliated withDepartment of Vascular Biology, Hirosaki University School of Medicine
  • , Etsuro ItoAffiliated withDepartment of Pediatrics, Hirosaki University School of Medicine
  • , Hiroshi TanakaAffiliated withDepartment of Pediatrics, Hirosaki University School of Medicine

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Abstract

Recent studies have suggested that gene expression studies using urinary sediment might be a non-invasive approach to assessing activity and pathogenesis in glomerulonephritis. However, little information is available regarding the mRNA expression patterns of functional molecules, such as T-bet, GATA-3, FOXP3, and retinoic acid-inducible gene-I (RIG-I), in urinary sediment, from patients with immunocomplex-mediated glomerulonephritis. Fourteen lupus nephritis (LN) patients, 13 IgA nephropathy (IgAN) patients, and 12 healthy controls were enrolled in the study. The mRNA expressions of T-bet, GATA-3, FOXP3 and RIG-I in urinary sediment were measured using real time quantitative polymerase chain reaction. We also studied the expression of RIG-I in kidney tissue specimens obtained from LN and IgAN patients. Significant differences in the expression patterns of GATA-3, FOXP3 and RIG-I, and marginal differences in T-bet expression, were observed between the three study groups. Immunofluorescent staining for RIG-I was observed in the tissue specimens from the LN patients, but not in those from the IgAN patients. The mRNA expression patterns of T-bet, GATA-3, FOXP3 and RIG-I in urinary sediment differ according to diagnostic category. These results suggest that the measurement of these target gene expressions might be a useful, non-invasive method for clinical monitoring and studying of pathogenesis in glomerulonephritis.

Keywords

Th1/Th2 balance T-bet GATA-3 FOXP3 RIG-I Urinary sediment Lupus nephritis IgA nephropathy