Article

Surgical Endoscopy

, Volume 27, Issue 3, pp 872-879

Open Access This content is freely available online to anyone, anywhere at any time.

Evaluation of lymphocytes CD4+ and CD8+ and expression of ZAP-70 kinase on CD3+ and CD19+ lymphocytes in obese patients undergoing laparoscopic cholecystectomy

  • Kamil TorresAffiliated withLaboratory of Biostructure, Human Anatomy Department, Medical University of LublinDepartment of General and Oncological Surgery, District Specialist Hospital Email author 
  • , Anna TorresAffiliated withLaboratory of Biostructure, Human Anatomy Department, Medical University of Lublin
  • , Andrzej ChrościckiAffiliated withDepartment of General and Oncological Surgery, District Specialist Hospital
  • , Ryszard MaciejewskiAffiliated withLaboratory of Biostructure, Human Anatomy Department, Medical University of Lublin
  • , Sebastian RadejAffiliated withLaboratory of Biostructure, Human Anatomy Department, Medical University of Lublin
  • , Jacek RolińskiAffiliated withDepartment of Clinical Immunology, Medical University of Lublin
  • , Łukasz PietrzykAffiliated withLaboratory of Biostructure, Human Anatomy Department, Medical University of Lublin
  • , Grzegorz WallnerAffiliated withDepartment of General and Gastroenterological Surgery, and Surgical Oncology of the Alimentary Tract, Medical University of Lublin

Abstract

Background

Obesity has become a global epidemic and a leading metabolic disease in the world. Laparoscopic surgeries may influence the function of the immunologic system. The percentages of CD4+ and CD8+ T lymphocyte cells have been described as prognostic factors for patients undergoing abdominal surgeries. This study aimed to evaluate the changes in CD4+ and CD8+ T lymphocyte cells, the ratio of CD4+ to CD8+ cells, and the ZAP-70 kinase expression on T CD3+ and B CD19+ cells in obese and normal-weight individuals undergoing laparoscopic cholecystectomy (LC).

Methods

The study group consisted of 46 asymptomatic patients with gallstones shown by ultrasound examination but without signs of any gallbladder complications. The patients underwent planned LC. Blood samples were obtained at three times, and the percentages of studied cells were measured by flow cytometry. Patients were enrolled to two groups: N group (body mass index [BMI], ≤25 kg/m2) and O group (BMI, ≥30 kg/m2). For statistical analysis, the Mann–Whitney U test and the Wilcoxon matched-pairs signed-ranks test were used. All p values lower than 0.05 were considered significant.

Results

The percentage of CD4+ T cells did not differ between the N and O groups before or after the surgery. Only in the N group did the percentage of CD4+ lymphocytes increase from 0 to 48 h. A higher percentage of CD8+ lymphocytes was observed in the O group postoperatively than in the N group. Differences of ZAP-70 kinase expression in the O group were observed at 24 and 48 h of the study. Decreased expression of ZAP-70 kinase was shown in the N group at both 0–24 and 24–48 h. In the O group, this tendency was noted at 24–48 h.

Conclusions

Immunologic activation after LC was confirmed in both weight groups. However, higher modulation, more typical for open surgeries, was observed in the obese group.

Keywords

Laparoscopy Obesity Pneumoperitoneum T cells ZAP-70 kinase CD4+ CD8+