Bioprocess Engineering

, Volume 18, Issue 5, pp 397–399

Poly(3-hydroxybutyrate) production from xylose by recombinant Escherichia coli

  • Sang Yup Lee

DOI: 10.1007/s004490050462

Cite this article as:
Yup Lee, S. Bioprocess Engineering (1998) 18: 397. doi:10.1007/s004490050462


Several recombinant Escherichia coli strains harboring the Alcaligenes eutrophus polyhydroxyalkanoate biosynthesis genes were used to produce poly(3-hydroxybutyrate), PHB, from xylose. By flask culture of TG1 (pSYL107) in a defined medium containing 20 g/l xylose, PHB concentration of 1.7 g/l was obtained. Supplementation of a small amount of cotton seed hydrolysate or soybean hydrolysate could enhance PHB production by more than two fold. The PHB concentration, PHB content, and PHB yield on xylose obtained by supplementing soybean hydrolysate were 4.4 g/l, 73.9%, and 0.226 g PHB/g xylose, respectively.

Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • Sang Yup Lee
    • 1
  1. 1.Department of Chemical Engineering and BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology (KAIST), 373-1 Kusong-dong, Yusong-gu, Taejon 305-701, Korea e-mail: