Bioprocess Engineering

, Volume 16, Issue 5, pp 261–264

Electroporation: basic principles, practical considerations and applications in molecular biology

  • G. Lakshmi Prasanna
  • T. Panda

DOI: 10.1007/s004490050319

Cite this article as:
Prasanna, G. & Panda, T. Bioprocess Engineering (1997) 16: 261. doi:10.1007/s004490050319


There are many approaches available for introducing foreign DNA into eukaryotes and prokaryotes. The most commonly used technique and currently of more practical interest is the electroporation. This review will focus on the electroporation as a promising, highly efficient and effective means of gene transfer. We summarize a detailed assessment of the various parameters and conditions that govern electroporation of a wide range of cell types.

Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • G. Lakshmi Prasanna
    • 1
  • T. Panda
    • 1
  1. 1.Biochemical Engineering Division, Department of Chemical Engineering, Indian Institute of Technology, Madras 600 036, IndiaIN

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