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Designing a gelatin/chitosan/hyaluronic acid biopolymer using a thermophysical approach for use in tissue engineering

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Abstract

Cell culture on biopolymeric scaffolds has provided treatments for tissue engineering. Biopolymeric mixtures based on gelatin (Ge), chitosan (Ch) and hyaluronic acid (Ha) have been used to make scaffolds for wound healing. Thermal and physical properties of scaffolds prepared with Ge, Ch and Ha were characterized. Thermal characterization was made by using differential scanning calorimetry (DSC), and physical characterization by gas pycnometry and scanning electron microscopy. The effects of Ge content and cross-linking on thermophysical properties were evaluated by means of a factorial experiment design (central composite face centered). Gelatin content was the main factor that affects the thermophysical properties (microstructure and thermal transitions) of the scaffold. The effect of Ge content of the scaffolds for tissue engineering was studied by seeding skin cells on the biopolymers. The cell attachment was not significantly modified at different Ge contents; however, the cell growth rate increased linearly with the decrease of the Ge content. This relationship together with the thermophysical characterization may be used to design scaffolds for tissue engineering.

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Acknowledgments

The authors wish to thank CONICYT from Chile for FONDECYT Grant 1120166.

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Authors and Affiliations

  1. Faculty of Medicine, School of Nutrition and Dietetics, School of Service Management, Universidad de los Andes, San Carlos de Apoquindo 2200, Las Condes, Santiago, Chile

    Javier Enrione & Paulo Díaz-Calderón

  2. Departamento de Ciencia y Tecnología de los Alimentos, Facultad Tecnológica, Universidad de Santiago de Chile (USACH), Avenida Ecuador 3769, Santiago, Chile

    Paulo Díaz-Calderón

  3. Departamento de Bioquímica, Facultad de Farmacia, Universidad de Valparaíso, Avenida Gran Bretaña 1093, Valparaíso, Chile

    Caroline R. Weinstein-Oppenheimer

  4. Centro de Biotecnología, Universidad Técnica Federico Santa María, Avenida España 1680, Valparaíso, Chile

    Elizabeth Sánchez, Miguel A. Fuentes & Cristian A. Acevedo

  5. Departamento de Biología y Ciencias Ambientales, Facultad de Ciencias, Universidad de Valparaíso, Avenida Gran Bretaña 1111, Valparaíso, Chile

    Donald I. Brown & Hugo Herrera

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  1. Javier Enrione
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  2. Paulo Díaz-Calderón
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  3. Caroline R. Weinstein-Oppenheimer
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  4. Elizabeth Sánchez
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  5. Miguel A. Fuentes
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  6. Donald I. Brown
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  7. Hugo Herrera
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  8. Cristian A. Acevedo
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Correspondence to Cristian A. Acevedo.

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Enrione, J., Díaz-Calderón, P., Weinstein-Oppenheimer, C.R. et al. Designing a gelatin/chitosan/hyaluronic acid biopolymer using a thermophysical approach for use in tissue engineering. Bioprocess Biosyst Eng 36, 1947–1956 (2013). https://doi.org/10.1007/s00449-013-0971-x

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  • DOI: https://doi.org/10.1007/s00449-013-0971-x

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