Bioprocess and Biosystems Engineering

, Volume 36, Issue 7, pp 885–892

Synthesis of nylon 4 from gamma-aminobutyrate (GABA) produced by recombinant Escherichia coli

  • Si Jae Park
  • Eun Young Kim
  • Won Noh
  • Young Hoon Oh
  • Hye Young Kim
  • Bong Keun Song
  • Kwang Myung Cho
  • Soon Ho Hong
  • Seung Hwan Lee
  • Jonggeon Jegal
Original Paper

DOI: 10.1007/s00449-012-0821-2

Cite this article as:
Park, S.J., Kim, E.Y., Noh, W. et al. Bioprocess Biosyst Eng (2013) 36: 885. doi:10.1007/s00449-012-0821-2

Abstract

In this study, we developed recombinant Escherichia coli strains expressing Lactococcus lactis subsp. lactis Il1403 glutamate decarboxylase (GadB) for the production of GABA from glutamate monosodium salt (MSG). Syntheses of GABA from MSG were examined by employing recombinant E. coli XL1-Blue as a whole cell biocatalyst in buffer solution. By increasing the concentration of E. coli XL1-Blue expressing GadB from the OD600 of 2–10, the concentration and conversion yield of GABA produced from 10 g/L of MSG could be increased from 4.3 to 4.8 g/L and from 70 to 78 %, respectively. Furthermore, E. coli XL1-Blue expressing GadB highly concentrated to the OD600 of 100 produced 76.2 g/L of GABA from 200 g/L of MSG with 62.4 % of GABA yield. Finally, nylon 4 could be synthesized by the bulk polymerization using 2-pyrrolidone that was prepared from microbially synthesized GABA by the reaction with Al2O3 as catalyst in toluene with the yield of 96 %.

Keywords

Gamma-aminobutyrate (GABA)MSGBioconversionRecombinant E. coliGlutamate decarboxylase2-PyrrolodineNylon 4

Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Si Jae Park
    • 1
    • 2
  • Eun Young Kim
    • 3
  • Won Noh
    • 3
  • Young Hoon Oh
    • 3
  • Hye Young Kim
    • 3
  • Bong Keun Song
    • 3
  • Kwang Myung Cho
    • 4
  • Soon Ho Hong
    • 5
  • Seung Hwan Lee
    • 3
  • Jonggeon Jegal
    • 3
  1. 1.Department of Environmental Engineering and Energy (Undergraduate program)Myongji UniversityYongin-siRepublic of Korea
  2. 2.Department of Energy Science and Technology (Graduate program)Myongji UniversityYongin-siRepublic of Korea
  3. 3.Industrial Biochemicals Research Group, Research Center for Biobased Chemistry, Division of Convergence ChemistryKorea Research Institute of Chemical TechnologyDaejeonRepublic of Korea
  4. 4.Easel BiotechnologiesLos AngelesUSA
  5. 5.School of Chemical Engineering and BioengineeringUniversity of UlsanUlsanRepublic of Korea