Original Paper

Bioprocess and Biosystems Engineering

, Volume 36, Issue 4, pp 433-441

Determining the transport mechanism of an enzyme-catalytic complex metabolic network based on biological robustness

  • Lei WangAffiliated withSchool of Mathematical Science, Dalian University of Technology Email author 

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Abstract

Understanding the transport mechanism of 1,3-propanediol (1,3-PD) is of critical importance to do further research on gene regulation. Due to the lack of intracellular information, on the basis of enzyme-catalytic system, using biological robustness as performance index, we present a system identification model to infer the most possible transport mechanism of 1,3-PD, in which the performance index consists of the relative error of the extracellular substance concentrations and biological robustness of the intracellular substance concentrations. We will not use a Boolean framework but prefer a model description based on ordinary differential equations. Among other advantages, this also facilitates the robustness analysis, which is the main goal of this paper. An algorithm is constructed to seek the solution of the identification model. Numerical results show that the most possible transport way is active transport coupled with passive diffusion.

Keywords

Complex metabolic network Biological robustness System identification Transport mechanism Optimal algorithm