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Use of focussed beam reflectance measurement (FBRM) for monitoring changes in biomass concentration

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An Erratum to this article was published on 02 August 2012

Abstract

The potential of focussed beam reflectance measurement (FBRM) as a tool to monitor changes in biomass concentration was investigated in a number of biological systems. The measurement technique was applied to two morphologically dissimilar plant cell suspension cultures, Morinda citrifolia and Centaurea calcitrapa, to a filamentous bacteria, Streptomyces natalensis, to high density cultures of Escherichia coli and to a murine Sp2/0 hybridoma suspension cell line, 3–2.19. In all cases, the biomass concentration proved to be correlated with total FBRM counts. The nature of the correlation varied between systems and was influenced by the concentration, nature, size and morphology of the particle under investigation.

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References

  1. Zhou WC, Hu WS (1994) Online characterization of a hybridoma cell culture process. Biotechnol Bioeng 44(2):170–177

    Article  CAS  Google Scholar 

  2. Junker BH, Reddy J, Gbewonyo K, Greasham R (1994) Online and in situ monitoring technology for cell density measurement in microbial and animal cell cultures. Bioprocess Eng 10(5–6):195–207

    Google Scholar 

  3. Wu P, Ozturk SS, Blackie JD, Thrift JC, Figueroa C, Naveh D (1995) Evaluation and applications of optical cell density probes in mammalian cell bioreactors. Biotechnol Bioeng 45(6):495–502

    Article  CAS  Google Scholar 

  4. Macmichael G, Armiger WB, Lee JF, Mutharasan R (1987) On-line measurement of hybridoma growth by culture fluorescence. Biotechnol techniques 1:213–218

    Article  Google Scholar 

  5. Kilburn DG, Fitzpatrick P, Blakecoleman BC, Clarke DJ, Griffiths JB (1989) Online monitoring of cell mass in mammalian cell cultures by acoustic densitometry. Biotechnol Bioeng 33(11):1379–1384

    Article  CAS  Google Scholar 

  6. Burgemeister S, Nattkemper TW, Noll T, Hoffrogge R, Flaschel E (2010) CellViCAM—Cell viability classification for animal cell cultures using dark filed micrographs. J Biotechnol 149(4):310–316

    Article  CAS  Google Scholar 

  7. Guez JS, Cassar JP, Wartelle F, Dhulster P, Suhr H (2010) The viability of animal cell cultures: can it be estimated online by using in situ microscopy? Process Biochem 45(2):288–291

    Article  CAS  Google Scholar 

  8. Wlaschin KF, Hu WS (2006) Fedbatch culture and dynamic nutrient feeding. Cell Culture Eng 101:43–74

    Article  CAS  Google Scholar 

  9. Barrett M, McNamara M, Hao HX, Barrett P, Glennon B (2010) Supersaturation tracking for the development, optimization and control of crystallization processes. Chem Eng Res Des 88(8A):1108–1119

    Article  CAS  Google Scholar 

  10. O’Grady D, Barrett M, Casey E, Glennon B (2007) The effect of mixing on the metastable zone width and nucleation kinetics in the anti-solvent crystallization of benzoic acid. Chem Eng Res Des 85(A7):945–952

    Article  Google Scholar 

  11. Barrett P, Glennon B (2002) Characterizing the metastable zone width and solubility curve using Lasentec FBRM and PVM. Chem Eng Res Des 80(A7):799–805

    Article  CAS  Google Scholar 

  12. Uduman N, Qi Y, Danquah MK, Hoadley AFA (2010) Marine microalgae flocculation and focused beam reflectance measurement. Chem Eng J 162(3):935–940

    Article  CAS  Google Scholar 

  13. Ge XM, Zhao XQ, Bai FW (2005) Online monitoring and characterization of flocculating yeast cell flocs during continuous ethanol fermentation. Biotechnol Bioeng 90(5):523–531

    Article  CAS  Google Scholar 

  14. McDonald KA, Jackman AP, Hurst S (2001) Characterization of plant suspension cultures using the focused beam reflectance technique. Biotechnol Lett 23(4):317–324

    Article  CAS  Google Scholar 

  15. Pearson AP, Glennon B, Kieran PM (2004) Monitoring of cell growth using the focused beam reflectance method. J Chem Technol Biotechnol 79(10):1142–1147

    Article  CAS  Google Scholar 

  16. Jeffers P, Raposo S, Lima-Costa ME, Connolly P, Glennon B, Kieran PM (2003) Focussed beam reflectance measurement (FBRM) monitoring of particle size and morphology in suspension cultures of Morinda citrifolia and Centaurea calcitrapa. Biotechnol Lett 25(23):2023–2028

    Article  CAS  Google Scholar 

  17. Sparks RG, Dobbs CL (1993) The use of laser backscatter instrumentation for the online measurement of the particle size distribution of emulsions. Part Part Syst Charact 10(5):279–289

    Article  CAS  Google Scholar 

  18. Hopfner T, Bluma A, Rudolph G, Lindner P, Scheper T (2010) A review of non-invasive optical-based image analysis systems for continuous bioprocess monitoring. Bioprocess Biosyst Eng 33(2):247–256

    Article  Google Scholar 

  19. Pearson AP, Glennon B, Kieran PM (2003) Comparison of morphological characteristics of Streptomyces natalensis by image analysis and focused beam reflectance measurement. Biotechnol Prog 19(4):1342–1347

    Article  CAS  Google Scholar 

  20. Seah SYK, Britton KL, Baker PJ, Rice DW, Asano Y, Engel PC (1995) Alteration in relative activities of phenylalanine dehydrogenase towards different substrates by site-directed mutagenesis. FEBS Lett 370(1–2):93–96

    Article  CAS  Google Scholar 

  21. Faulkner E, Barrett M, Okor S, Kieran P, Casey E, Paradisi F, Engel P, Glennon B (2006) Use of fed-batch cultivation for achieving high cell densities for the pilot-scale production of a recombinant protein (phenylalanine dehydrogenase) in Escherichia coli. Biotechnol Prog 22(3):889–897

    Article  CAS  Google Scholar 

  22. Fitzpatrick B, O’Kennedy R (2004) The development and application of a surface plasmon resonance-based inhibition immunoassay for the determination of warfarin in plasma ultrafiltrate. J Immunol Methods 291(1–2):11–25

    Article  CAS  Google Scholar 

  23. Barrett P (2002) In situ monitoring of crystallization processes. University College Dublin, Dublin

    Google Scholar 

  24. Heath AR, Fawell PD, Bahri PA, Swift JD (2002) Estimating average particle size by focused beam reflectance measurement (FBRM). Part Part Syst Charact 19(2):84–95

    Article  Google Scholar 

  25. Emery AN, Jan DCH, Al-Rubeai M (1995) Oxygenation of intensive cell culture system. Appl Microb Biotechnol 43(6):1028–1033

    Article  CAS  Google Scholar 

  26. Ishaque A, Al-Rubeai M (2004) Monitoring of apoptosis. In: Al-Rubeai M, Fussenegger M (eds) Cell engineering: apoptosis, vol 4. Springer, Heidelberg, pp 281–306

    Google Scholar 

  27. Van de Goor J (2004) Improvement of industrial cell culture processes by caspase-9 dominant negative and other apoptosis inhibitors. In: Al-Rubeai M, Fussenegger M (eds) Cell engineering: apoptosis, vol 4. Springer, Heidelberg, pp 211–221

    Google Scholar 

  28. Zeng A, Bi J (2005) Cell culture kinetics and modeling. In: Ozturk SS, Hu WS (eds) Cell culture technology for pharmaceutical and cell-based therapies. CRC Press, Boca Raton, pp 299–348

    Google Scholar 

  29. Franek F, Dolnikova J (1991) Hybridoma growth and monoclonal antibody production in iron-rich protein-free medium: effect of nutrient concentration. Cytotechnology 7(1):33–38

    Article  CAS  Google Scholar 

  30. Mercille S, Massie B (1994) Induction of apoptosis in nutrient-deprived cultures of hybridoma and myeloma cells. Biotechnol Bioeng 44(9):1140–1154

    Article  CAS  Google Scholar 

  31. Singh RP, Al-Rubeai M, Gregory CD, Emery AN (1994) Cell death in bioreactors: a role for apoptosis. Biotechnol Bioeng 44(6):720–726

    Article  CAS  Google Scholar 

  32. Bartlett M, Huang G, Larcom L, Jiang HB (2004) Measurement of particle size distribution in mammalian cells in vitro by use of polarized light spectroscopy. Appl Opt 43:1296–1307

    Article  Google Scholar 

  33. Backman V, Gopal V, Kalashnikov M, Badizadegan K, Gurjar R, Wax A, Georgakoudi I, Mueller M, Boone CW, Dasari RR, Feld MS (2001) Measuring cellular structure at submicrometer scale with light scattering spectroscopy. IEEE J Sel Topics Quantum Electron 7(6):887–893

    Article  CAS  Google Scholar 

  34. Gurjar RS, Backman V, Perelman LT, Georgakoudi I, Badizadegan K, Itzkan I, Dasari RR, Feld MS (2001) Imaging human epithelial properties with polarized light-scattering spectroscopy. Nat Med 7(11):1245–1248

    Article  CAS  Google Scholar 

  35. Drezek R, Guillaud M, Collier T, Boiko I, Malpica A, Macaulay C, Follen M, Richards-Kortum R (2003) Light scattering from cervical cells throughout neoplastic progression: influence of nuclear morphology, DNA content, and chromatin texture. J Biomedical Opt 8(1):7–16

    Article  Google Scholar 

  36. Collins RJ, Harmon BV, Gobe GC, Kerr JFR (1992) Inter nucleosomal DNA cleavage should not be the sole criterion for identifying apoptosis. Int J Radiat Biol 61(4):451–453

    Article  CAS  Google Scholar 

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Acknowledgments

The authors gratefully acknowledge the financial support of the following: DPS Engineering Ltd., Enterprise Ireland, UCD and IRCSET, the Irish Research Council for Science, Engineering and Technology.

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Authors and Affiliations

  1. UCD School of Chemical and Bioprocess Engineering, UCD, Belfield, Dublin 4, Ireland

    Jessica Whelan, Patricia Kieran, Susan McDonnell & Brian Glennon

  2. Training Department, National Institute of Bioprocessing Research and Training, Foster’s Avenue, Mount Merrion, Blackrock, Dublin 4, Ireland

    Eilis Murphy

  3. Pharmaceutical Operations, MSD Ireland, Clonmel, Tipperary, Ireland

    Alan Pearson

  4. Process Analytical Sciences Group, Pfizer Ireland Pharmaceuticals, Loughbeg, Cork, Ireland

    Paul Jeffers

Authors
  1. Jessica Whelan
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  2. Eilis Murphy
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  3. Alan Pearson
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  4. Paul Jeffers
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  5. Patricia Kieran
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  6. Susan McDonnell
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  7. Brian Glennon
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Correspondence to Brian Glennon.

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Whelan, J., Murphy, E., Pearson, A. et al. Use of focussed beam reflectance measurement (FBRM) for monitoring changes in biomass concentration. Bioprocess Biosyst Eng 35, 963–975 (2012). https://doi.org/10.1007/s00449-012-0681-9

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  • DOI: https://doi.org/10.1007/s00449-012-0681-9

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