Bioprocess and Biosystems Engineering

, Volume 35, Issue 1, pp 49–54

Effects of deletion of glycerol-3-phosphate dehydrogenase and glutamate dehydrogenase genes on glycerol and ethanol metabolism in recombinant Saccharomyces cerevisiae

Original Paper

DOI: 10.1007/s00449-011-0590-3

Cite this article as:
Kim, JW., Chin, YW., Park, YC. et al. Bioprocess Biosyst Eng (2012) 35: 49. doi:10.1007/s00449-011-0590-3


Bioethanol is currently used as an alternative fuel for gasoline worldwide. For economic production of bioethanol by Saccharomyces cerevisiae, formation of a main by-product, glycerol, should be prevented or minimized in order to reduce a separation cost of ethanol from fermentation broth. In this study, S. cerevisiae was engineered to investigate the effects of the sole and double disruption of NADH-dependent glycerol-3-phosphate dehydrogenase 1 (GPD1) and NADPH-requiring glutamate dehydrogenase 1 (GDH1) on the production of glycerol and ethanol from glucose. Even though sole deletion of GPD1 or GDH1 reduced glycerol production, double deletion of GPD1 and GDH1 resulted in the lowest glycerol concentration of 2.31 g/L, which was 46.4% lower than the wild-type strain. Interestingly, the recombinant S. cerevisiae∆GPD1∆GDH1 strain showed a slight improvement in ethanol yield (0.414 g/g) compared with the wild-type strain (0.406 g/g). Genetic engineering of the glycerol and glutamate metabolic pathways modified NAD(P)H-requiring metabolic pathways and exerted a positive effect on glycerol reduction without affecting ethanol production.


Saccharomyces cerevisiaeEthanolGlycerolGlycerol-3-phosphate dehydrogenase 1 (GPD1)Glutamate dehydrogenase 1 (GDH1)

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  1. 1.Department of Agricultural BiotechnologySeoul National UniversitySeoulKorea
  2. 2.Department of Advanced Fermentation Fusion Science and TechnologyKookmin UniversitySeoulKorea