Original Paper

Bioprocess and Biosystems Engineering

, Volume 35, Issue 3, pp 351-358

First online:

Assessment of two immobilized lipases activity and stability to low temperatures in organic solvents under ultrasound-assisted irradiation

  • Luciane BatistellaAffiliated withDepartamento de Engenharia de Alimentos, Universidade Regional Integrada do Alto Uruguai e das Missões, URI—Campus de Erechim
  • , Mara K. UstraAffiliated withDepartamento de Engenharia de Alimentos, Universidade Regional Integrada do Alto Uruguai e das Missões, URI—Campus de Erechim
  • , Aline RichettiAffiliated withDepartamento de Engenharia de Alimentos, Universidade Regional Integrada do Alto Uruguai e das Missões, URI—Campus de Erechim
  • , Sibele B. C. PergherAffiliated withDepartamento de Química, Universidade Federal do Rio Grande do Norte, Centro de Ciências Exatas
  • , Helen TreichelAffiliated withDepartamento de Engenharia de Alimentos, Universidade Regional Integrada do Alto Uruguai e das Missões, URI—Campus de Erechim
  • , J. V. OliveiraAffiliated withDepartamento de Engenharia de Alimentos, Universidade Regional Integrada do Alto Uruguai e das Missões, URI—Campus de Erechim
  • , Lindomar LerinAffiliated withDepartamento de Engenharia de Alimentos, Universidade Regional Integrada do Alto Uruguai e das Missões, URI—Campus de Erechim
  • , Débora de OliveiraAffiliated withDepartamento de Engenharia de Alimentos, Universidade Regional Integrada do Alto Uruguai e das Missões, URI—Campus de Erechim Email author 

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Abstract

Both stability and catalytic activity of two commercial immobilized lipases were investigated in the presence of different organic solvents in ultrasound-assisted system. In a general way, for Novozym 435, the use of ethanol as solvent led to a loss of activity of 35% after 10 h of contact. The use of iso-octane conducted to a gradual increase in lipase activity in relation to the contact time, reaching a maximum value of relative activity of 126%. For Lipozyme RM IM, after 5 h of exposure, the enzyme presented no residual activity when ethanol was used as solvent. The solvents tert-butanol and iso-octane showed an enhancement of about 20 and 17% in the enzyme activity in 6 h of exposure, respectively. Novozym 435 and Lipozyme IM presented high stability to storage after treatment under ultrasound-assisted system using n-hexane and tert-butanol as solvents.

Keywords

Lipases Enzyme activity Ultrasound system Enzyme stability