Bioprocess and Biosystems Engineering

, 33:21

In vitro cytotoxicity screening of water-dispersible metal oxide nanoparticles in human cell lines

Authors

  • Jong Young Choi
    • Department of Chemical and Biomolecular Engineering (BK21 Program)Institute for the BioCentury, KAIST
  • Su Hee Lee
    • Department of Chemical and Biomolecular Engineering (BK21 Program)Institute for the BioCentury, KAIST
  • Hyon Bin Na
    • National Creative Research Initiative Center for Oxide Nanocrystalline Materials, School of Chemical and Biological EngineeringSeoul National University
  • Kwangjin An
    • National Creative Research Initiative Center for Oxide Nanocrystalline Materials, School of Chemical and Biological EngineeringSeoul National University
  • Taeghwan Hyeon
    • National Creative Research Initiative Center for Oxide Nanocrystalline Materials, School of Chemical and Biological EngineeringSeoul National University
    • Department of Chemical and Biomolecular Engineering (BK21 Program)Institute for the BioCentury, KAIST
Original Paper

DOI: 10.1007/s00449-009-0354-5

Cite this article as:
Choi, J.Y., Lee, S.H., Na, H.B. et al. Bioprocess Biosyst Eng (2010) 33: 21. doi:10.1007/s00449-009-0354-5

Abstract

In this study, we present in vitro cytotoxicity of iron oxide (Fe3O4) and manganese oxide (MnO) using live/dead cell assay, lactate dehydrogenase assay, and reactive oxygen species detection with variation of the concentration of nanoparticles (5–500 μg/ml), incubation time (18–96 h), and different human cell lines (lung adenocarcinoma, breast cancer cells, and glioblastoma cells). The surface of nanoparticles is modified with polyethyleneglycol-derivatized phospholipid to enhance the biocompatibility, water-solubility, and stability under an aqueous media. While the cytotoxic effect was negligible for 18 h incubation even at highest concentration of 500 μg/ml, MnO nanoparticle represented higher level of toxicity than those of Fe3O4 and the commercial medical contrast reagent, Feridex after 2 and 4 day incubation time. However, the cytotoxicity of Fe3O4 is equivalent or better than Feridex based on the live/dead cell viability assay. The engineered MnO and Fe3O4 exhibited excellent stability compared with Feridex for a prolonged incubation time.

Keywords

Water-dispersible Fe3O4 and MnO nanoparticleMagnetic resonance imaging contrast agentsCytotoxicityNanotoxicology

Copyright information

© Springer-Verlag 2009