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Effect of aeration and agitation on the protease production by Staphylococcus aureus mutant RC128 in a stirred tank bioreactor

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Abstract

The modified rotating simplex method has been successfully used to determine the best combination of agitation rate and aeration rate for maximum production of extracellular proteases by Staphylococcus aureus mutant RC128, in a stirred tank bioreactor operated in a discontinuous way. This mutant has shown altered exoprotein production, specially enhanced protease production. Maximum production of proteases (15.28 UP/ml), measured using azocasein as a substrate, was obtained at exponential growth phase when the bioreactor was operated at 300 rpm and at 2 vvm with a volumetric oxygen transfer coefficient (K L a) of 175.75 h−1. These conditions were found to be more suitable for protease production.

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Acknowledgments

The authors wish to thank the support given by the Secretaría de Ciencia y Técnica Universidad Nacional de Río Cuarto (UNRC), Argentina.

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Authors and Affiliations

  1. Departamento de Tecnología Química, Facultad de Ingeniería, Universidad Nacional de Río Cuarto, Río Cuarto, Argentina

    E. Ducros & M. Ferrari

  2. Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Río Cuarto, Argentina

    M. Pellegrino, C. Raspanti & C. Bogni

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  1. E. Ducros
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  2. M. Ferrari
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  3. M. Pellegrino
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Correspondence to E. Ducros.

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Ducros, E., Ferrari, M., Pellegrino, M. et al. Effect of aeration and agitation on the protease production by Staphylococcus aureus mutant RC128 in a stirred tank bioreactor. Bioprocess Biosyst Eng 32, 143–148 (2009). https://doi.org/10.1007/s00449-008-0233-5

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  • DOI: https://doi.org/10.1007/s00449-008-0233-5

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