Modeling simultaneous glucose and xylose uptake in Saccharomyces cerevisiae from kinetics and gene expression of sugar transporters
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- Bertilsson, M., Andersson, J. & Lidén, G. Bioprocess Biosyst Eng (2008) 31: 369. doi:10.1007/s00449-007-0169-1
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A kinetic model for glucose and xylose co-substrate uptake in Saccharomyces cerevisiae is presented. The model couples the enzyme kinetics with the glucose-dependent genetic expression of the individual transport proteins. This novel approach implies several options for optimizing the co-substrate utilization. Interestingly, the simulations predict a maximum xylose uptake rate at a glucose concentration >0 g/L, which suggests that the genetic expressions of the considered transport proteins are of importance when optimizing the xylose uptake. This was also evident in fed-batch simulations, where a distinct optimal glucose addition rate >0 g/L·h was found. Strategies for improving the co-substrate utilization by genetic engineering of the transport systems are furthermore suggested based on simulations.