Bioprocess and Biosystems Engineering

, Volume 30, Issue 4, pp 231–241

Evaluation of a novel Wave Bioreactor® cellbag for aerobic yeast cultivation

Original Paper

DOI: 10.1007/s00449-007-0119-y

Cite this article as:
Mikola, M., Seto, J. & Amanullah, A. Bioprocess Biosyst Eng (2007) 30: 231. doi:10.1007/s00449-007-0119-y

Abstract

The Wave Bioreactor® is widely used in cell culture due to the benefits of disposable technology and ease of use. A novel cellbag was developed featuring a frit sparger to increase the system's oxygen transfer. The purpose of this work was to evaluate the sparged cellbag for yeast cultivation. Oxygen mass transfer studies were conducted in simulated culture medium and the sparged system's maximum oxygen mass transfer coefficient (kLa) was 38 h-1. These measurements revealed that the sparger was ineffective in increasing the oxygen transfer capacity. Cultures of Saccharomyces cerevisiae were successfully grown in oxygen-blended sparged and oxygen-blended standard cellbags. Under steady state conditions for both cellbag designs, kLa values as high as 60 h-1 were obtained with no difference in growth characteristics. This is the first report of a successful cultivation of a microbe in a Wave Bioreactor® comparing conventional seed expansion in shake flasks and stirred tank bioreactors.

Keywords

Wave Bioreactor Disposable bioreactor Saccharomyces cerevisiae Oxygen transfer Seed culture 

List of symbols

C*

saturated dissolved oxygen concentration in the liquid phase (mmol L−1)

C1

dissolved oxygen concentration at time t1 (mmol L−1)

C2

dissolved oxygen concentration at time t2 (mmol L−1)

CER

carbon dioxide evolution rate (mmol L−1 h−1)

DO

dissolved oxygen (% air sat)

kLa

volumetric oxygen transfer coefficient (h−1)

OD

optical density

OTR

oxygen transfer rate (mmol L−1 h−1)

OUR

oxygen uptake rate (mmol L−1 h−1)

PBS

phosphate buffered saline

RPM

rocks per minute (min−1)

RQ

respiration quotient (CER/OUR)

s

substrate (g L−1)

vvm

volume of gas sparge per volume liquid per minute (L L−1 min−1)

Yx/s

yield coefficient, g biomass/g glucose

x

biomass (g)

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  1. 1.Fermentation and Cell CultureMerck and Co.West PointUSA