Bioprocess and Biosystems Engineering

, Volume 30, Issue 1, pp 13–25

Feeding strategies for E. coli fermentations demanding an enriched environment

  • L. de Maré
  • C. Cimander
  • A. Elfwing
  • P. Hagander
Original Paper

DOI: 10.1007/s00449-006-0090-z

Cite this article as:
de Maré, L., Cimander, C., Elfwing, A. et al. Bioprocess Biosyst Eng (2007) 30: 13. doi:10.1007/s00449-006-0090-z

Abstract

The addition of a carbon nutrient feed to a fed-batch cultivation is often not enough to obtain satisfactory growth and/or production. In some cases, an additional feed with for example supplementary amino acids or complex media is required. This work presents the development of feeding strategies where more than one feed is required and the knowledge of the growth requirements is low. Simulations and cultivations with E. coli are shown using the proposed feed controllers which are based on a probing control concept. The strategies work well and they can be used to shorten the process development phase considerably.

Keywords

Fermentation processFeeding strategyFed-batch cultivationEscherichia coliComplex mediumAmino acid

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • L. de Maré
    • 1
  • C. Cimander
    • 2
  • A. Elfwing
    • 2
  • P. Hagander
    • 1
  1. 1.Department of Automatic ControlLTH, Lund UniversityLundSweden
  2. 2.Novozymes Biopharma ABLundSweden