Oecologia

, Volume 172, Issue 1, pp 21–34

Tissue turnover and stable isotope clocks to quantify resource shifts in anadromous rainbow trout

Physiological ecology - Original research

DOI: 10.1007/s00442-012-2483-9

Cite this article as:
Heady, W.N. & Moore, J.W. Oecologia (2013) 172: 21. doi:10.1007/s00442-012-2483-9

Abstract

Stable isotopes can illuminate resource usage by organisms, but effective interpretation is predicated on laboratory validation. Here we develop stable isotope clocks to track resource shifts in anadromous rainbow trout (Oncorhynchus mykiss). We used a diet-switch experiment and model fitting to quantify N stable isotope (δ15N) turnover rates and discrimination factors for seven tissues: plasma, liver, fin, mucus, red blood cells, muscle, and scales. Among tissues, diet-tissue δ15N discrimination factors ranged from 1.3 to 3.4 ‰. Model-supported tissue turnover half-lives ranged from 9.0 (fin) to 27.7 (scale) days. We evaluated six tissue turnover models using Akaike’s information criterion corrected for small sample sizes. The use of equilibrium tissue values was supported in all tissues and two-compartment models were supported in plasma, liver, and mucus. Using parameter estimates and their uncertainty we developed stable isotope clocks to estimate the time since resource shifts. Longer turnover tissues provided accurate estimates of time since resource switch for durations approximately twice their half-life. Faster turnover tissues provided even higher precision estimates, but only within their half-life post-switch. Averaging estimates of time since resource shift from multiple tissues provided the highest precision estimates of time since resource shift for the longest duration (up to 64 days). This study therefore provides insight into physiological processes that underpin stable isotope patterns, explicitly tests alternative models, and quantifies key parameters that are the foundation of field-based stable isotope analysis.

Keywords

Oncorhynchus mykissMigrationMixing modelNitrogenDiscrimination factor

Supplementary material

442_2012_2483_MOESM1_ESM.doc (1.3 mb)
Supplementary material 1 (DOC 1364 kb)

Copyright information

© Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  1. 1.Department of Ecology and Evolutionary BiologyUniversity of California Santa CruzSanta CruzUSA
  2. 2.Moss Landing Marine LaboratoriesMoss LandingUSA
  3. 3.Department of Biological Sciences, Earth2Ocean Research GroupSimon Fraser UniversityBurnabyCanada