Cell and Tissue Research

, Volume 306, Issue 2, pp 257–264

Identification of a novel, multifunctional β-defensin (human β-defensin 3) with specific antimicrobial activity

Its interaction with plasma membranes of Xenopus oocytes and the induction of macrophage chemoattraction
  • José-Ramón García
  • Florian Jaumann
  • Sandra Schulz
  • Alexander Krause
  • Javier Rodríguez-Jiménez
  • Ulf Forssmann
  • Knut Adermann
  • Enno Klüver
  • Claus Vogelmeier
  • Dirk Becker
  • Rainer Hedrich
  • Wolf-Georg Forssmann
  • Robert Bals
Regular Article

DOI: 10.1007/s004410100433

Cite this article as:
García, J., Jaumann, F., Schulz, S. et al. Cell Tissue Res (2001) 306: 257. doi:10.1007/s004410100433

Abstract.

Previous studies have shown the implication of β-defensins in host defense of the human body. The human β-defensins 1 and 2 (hBD-1, hBD-2) have been isolated by biochemical methods. Here we report the identification of a third human β-defensin, called human β-defensin 3 (hBD-3; cDNA sequence, Genbank accession no. AF295370), based on bioinformatics and functional genomic analysis. Expression of hBD-3 is detected throughout epithelia of many organs and in non-epithelial tissues. In contrast to hBD-2, which is upregulated by microorganisms or tumor necrosis factor-α (TNF-α), hBD-3 expression is increased particularly after stimulation by interferon-γ. Synthetic hBD-3 exhibits a strong antimicrobial activity against gram-negative and gram-positive bacteria and fungi, including Burkholderia cepacia. In addition, hBD-3 activates monocytes and elicits ion channel activity in biomembranes, specifically in oocytes of Xenopus laevis. This paper also shows that screening of genomic sequences is a valuable tool with which to identify novel regulatory peptides. Human β-defensins represent a family of antimicrobial peptides differentially expressed in most tissues, regulated by specific mechanisms, and exerting physiological functions not only related to direct host defense.

β-Defensin Burkholderia cepacia Cystic fibrosis Innate host defense Antimicrobial peptide Human

Copyright information

© Springer-Verlag 2001

Authors and Affiliations

  • José-Ramón García
    • 1
  • Florian Jaumann
    • 2
  • Sandra Schulz
    • 1
  • Alexander Krause
    • 1
  • Javier Rodríguez-Jiménez
    • 1
  • Ulf Forssmann
    • 1
  • Knut Adermann
    • 1
  • Enno Klüver
    • 1
  • Claus Vogelmeier
    • 2
  • Dirk Becker
    • 3
  • Rainer Hedrich
    • 3
  • Wolf-Georg Forssmann
    • 1
  • Robert Bals
    • 2
  1. 1.IPF Pharmaceuticals GmbH, Feodor-Lynen-Strasse 31, 30625 Hanover, Germany
  2. 2.Medizinische Klinik I, Grosshadern, Ludwig-Maximilians-Universität, 81377 Munich, Germany
  3. 3.Julius-von-Sachs-Institut für Biowissenschaften, Lehrstuhl für Botanik I, Molekulare Pflanzenphysiologie und Biophysik, 97082 Würzburg, Germany