Cell and Tissue Research

, Volume 304, Issue 2, pp 317–321

Localization of an insulin-like peptide in brains of two flies

  • Chun Cao
  • Mark R. Brown
Short Communication

DOI: 10.1007/s004410100367

Cite this article as:
Cao, C. & Brown, M. Cell Tissue Res (2001) 304: 317. doi:10.1007/s004410100367

Abstract.

A nucleotide sequence (GenBank AI134194) was identified in the database of the Berkeley Drosophila Genome Project based on the similarity of its translated sequence to insulin-like peptides known for other insects. The putative A chain of the Drosophila peptide was synthesized and used to produce an antiserum for immunocytochemistry. Medial neurosecretory cells and their axons were immunostained in whole brains of Drosophila larvae and adults. In larvae, immunostained axons from these cells extended to the corpus cardiacum in the ring gland and the hypocerebral ganglion and along the aorta in the head. In adults, axons from these cells extended along the aorta to the corpus cardiacum-hypocerebral ganglion complex near the cardiac valve and branched along the midgut and crop. In contrast, two clusters of lateral neurosecretory cells and their axons were immunostained weakly in brains of female mosquitoes, Aedes aegypti. No other cells were stained in the nervous systems or midguts of Drosophila larvae and adults or Ae. aegypti females. These specific localizations substantiate the existence of an endogenous insulin-like peptide in Drosophila and suggest that a similar peptide is present in the distantly related mosquitoes.

Neurosecretion Bombyxin Drosophila melagonaster Aedes aegypti (Insecta)

Copyright information

© Springer-Verlag 2001

Authors and Affiliations

  • Chun Cao
    • 1
  • Mark R. Brown
    • 1
  1. 1.Department of Entomology, University of Georgia, Athens, GA 30602, USA