Cell and Tissue Research

, Volume 295, Issue 2, pp 307–316

BDM (2,3-butanedione monoxime), an inhibitor of myosin-actin interaction, suppresses myofibrillogenesis in skeletal muscle cells in culture

Authors

  • Y. Soeno
    • Department of Biology, Faculty of Science, Chiba University, Yayoi-cho, Chiba 263-8522, Japan Tel.: +81 3 290 2804; Fax: +81 43 290 2807; e-mail: tobinata@nature.s.chiba-u.ac.jp
  • Y. Shimada
    • Department of Anatomy, School of Medicine, Chiba University, Inohana, Chiba 260-8670, Japan
  • T. Obinata
    • Department of Biology, Faculty of Science, Chiba University, Yayoi-cho, Chiba 263-8522, Japan Tel.: +81 3 290 2804; Fax: +81 43 290 2807; e-mail: tobinata@nature.s.chiba-u.ac.jp
REGULAR ARTICLE

DOI: 10.1007/s004410051237

Cite this article as:
Soeno, Y., Shimada, Y. & Obinata, T. Cell Tissue Res (1999) 295: 307. doi:10.1007/s004410051237
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Abstract

During the initial phase of myofibrillogenesis in developing muscle cells, the majority of thin filaments lie parallel to, and exhibit correct polarity and spatial position with thick filaments, as in mature myofibrils. Since myosin is known to function as an accelerator of actin polymerization in vitro, it has been postulated that myosin-actin interaction is important in the initial phase of myofibrillogenesis. To clarify further the role of actin-myosin interaction in myofibril formation during development, BDM (2,3-butanedione 2-monoxime), an inhibitor of myosin ATPase, was applied to primary cultures of skeletal muscle to inhibit myosin activity during myofibrillogenesis, and myofibril formation was examined. When 10 mM BDM was added to the myotubes just after fusion and the cultures were maintained for a further 4 days, cross-striated myofibrils were scarcely observed by fluorescence microscopy when examined by staining with antibodies to actin, myosin, troponin and α-actinin, whereas in the control myotubes not exposed to BDM, typical sarcomeric structures were detected. Electron microscopy revealed a disorganized arrangement of myofilaments and incomplete sarcomeric structures in the BDM-treated myotubes. Thus, formation of cross-striated myofibrils was remarkably suppressed in the BDM-treated myotubes. When the myotubes cultured in BDM-containing media were transferred to control media, sarcomeric structures were formed in 2–3 days, suggesting that the inhibitory effect of BDM on myotubes is reversible. These results suggest that actin-myosin interaction plays a critical role in the early process of myofibrillogenesis.

Key words Myofibril assemblyActinMyosin23-Butanedione 2-monoxime (BDM)Skeletal muscleCultureChicken
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© Springer-Verlag Berlin Heidelberg 1999