Cell and Tissue Research

, Volume 293, Issue 3, pp 445–452

Defective cytoplasmic granule formation

II. Differences in patterns of radiolabeling of secretory granules in beige versus normal mouse pancreatic acinar cells after [3H]glycine administration in vivo
  • I. Hammel
  • Ann M. Dvorak
  • Patricia Fox
  • Edith Shimoni
  • Stephen J. Galli
REGULAR ARTICLE

DOI: 10.1007/s004410051136

Cite this article as:
Hammel, I., Dvorak, A., Fox, P. et al. Cell Tissue Res (1998) 293: 445. doi:10.1007/s004410051136

Abstract 

We investigated the development of secretory granules in the pancreatic acinar cells of normal (C57BL/6J +/+) and beige (C57BL/6J Lystbg/Lystbg) mice by analyzing the distribution of 3H label in pancreatic acinar cells after a pulse of [3H]glycine administered in vivo. The results provide quantitative confirmation of the hypothesis that the maturation of condensing vacuoles/immature granules to mature granules in pancreatic acinar cells is associated with a significant volume reduction. Beige mice differ from control mice by exhibiting a more rapid distribution of 3H label from the rough endoplasmic reticulum-rich cytoplasm to the secretory granules and a slightly faster rate of maturation of 3H-labeled granules. Beige mouse pancreatic acinar cells also exhibited, as early as 1 h after pulsing with [3H]glycine, a much higher proportion of 3H-labeled secretory granules than did the cells of control mice. These findings identify additional abnormalities in secretory granule formation in pancreatic acinar cells which are related to the beige (Lystbg) mutation and provide support for the hypothesis that beige mice exhibit an abnormal pattern of granule-granule fusion.

Key words Chédiak-Higashi syndromeCondensing vacuoleGolgi complexUnit granule formationMouse (beige)

Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • I. Hammel
    • 1
  • Ann M. Dvorak
    • 2
  • Patricia Fox
    • 2
  • Edith Shimoni
    • 1
  • Stephen J. Galli
    • 2
  1. 1.Department of Pathology, Sackler Faculty of Medicine, Tel Aviv University, Ramat Aviv, Tel Aviv 69978, IsraelIL
  2. 2.Departments of Pathology, Harvard Medical School and Beth Israel Deaconess Medical Center, 330 Brookline Avenue, Boston, MA 02215, USAIL