Cell and Tissue Research

, Volume 293, Issue 2, pp 277–284

Ultrastructural characterization of interstitial cells of Cajal in the rat small intestine using control and Ws/Ws mutant rats

  • Kazuhide Horiguchi
  • T. Komuro

DOI: 10.1007/s004410051119

Cite this article as:
Horiguchi, K. & Komuro, T. Cell Tissue Res (1998) 293: 277. doi:10.1007/s004410051119


Interstitial cells in the myenteric plexus and the deep muscular plexus of the small intestine of the c-kit mutant rats (Ws/Ws) and their normal siblings (+/+) were studied. c-Kit immunoreactivity was detected in two regions corresponding to the myenteric plexus and the deep muscular plexus in the jejunum of +/+ rats, while no immunoreactivity was detected in Ws/Ws rats. Using electron microscopy, two types of gap junction-forming interstitial cells were found in association with the myenteric plexus in +/+ rats: one type characterized by a typical fibroblastic ultrastructure, and the other characterized by numerous mitochondria and less electron-dense cytoplasm. Since the latter were greatly reduced in Ws/Ws rats, it was suggested that these cells correspond to c-kit-expressing cells, i.e. interstitial cells of Cajal in the myenteric plexus region. In contrast, two types of interstitial cells in the region of the deep muscular plexus were observed with no difference between +/+ and Ws/Ws rats. Probable interstitial cells of Cajal in this region were characterized by a basal lamina and numerous caveolae as well as large gap junctions that interconnect with each other and with the smooth muscle cells. We concluded that interstitial cells of Cajal in the rat intestine are heterogeneous in ultrastructure, c-kit dependency in the cell maturation, and functional role.

Key words Interstitial cells of Cajal (ICC) c-Kit Small intestine pacemaker Morphology Rat (Ws/Ws) 

Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • Kazuhide Horiguchi
    • 1
  • T. Komuro
    • 1
  1. 1.Department of Basic Human Sciences, School of Human Sciences, Waseda University, Tokorozawa, Saitama, 359 Japan Fax:+81 429 48 4314; e-mail: komuro@human.waseda.ac.jpJP

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