Cell and Tissue Research

, Volume 292, Issue 3, pp 597–607

Cellular origin of chlorinated diketopiperazines in the dictyoceratid sponge Dysidea herbacea (Keller)

Authors

  • Andrew E. Flowers
    • Department of Chemistry, The University of Queensland, Brisbane QLD 4072, Australia Fax: +61–7–3365–4299; E-mail garson@chemistry.uq.edu.au
  • M. J. Garson
    • Department of Chemistry, The University of Queensland, Brisbane QLD 4072, Australia Fax: +61–7–3365–4299; E-mail garson@chemistry.uq.edu.au
  • Richard I. Webb
    • Centre for Microscopy and Microanalysis and Department of Microbiology, The University of Queensland, Brisbane QLD 4072, Australia
  • Eric J. Dumdei
    • Department of Chemistry, The University of Queensland, Brisbane QLD 4072, Australia Fax: +61–7–3365–4299; E-mail garson@chemistry.uq.edu.au
  • Romila D. Charan
    • Department of Chemistry, The University of Queensland, Brisbane QLD 4072, Australia Fax: +61–7–3365–4299; E-mail garson@chemistry.uq.edu.au
REGULAR ARTICLE

DOI: 10.1007/s004410051089

Cite this article as:
Flowers, A., Garson, M., Webb, R. et al. Cell Tissue Res (1998) 292: 597. doi:10.1007/s004410051089

Abstract 

The tropical marine sponge Dysidea herbacea (Keller) contains the filamentous unicellular cyanobacterium Oscillatoria spongeliae (Schulze) Hauck as an endosymbiont, plus numerous bacteria, both intracellular and extracellular. Archaeocytes and choanocytes are the major sponge cell types present. Density gradient centrifugation of glutaraldehyde-fixed cells with Percoll as the support medium has been used to separate the cyanobacterial symbiont from the sponge cells on the basis of their differing densities. The protocol also has the advantage of separating broken from intact cells of O. spongeliae. The lighter cell preparations contain archaeocytes and choanocytes together with damaged cyanobacterial cells, whereas heavier cell preparations contain intact cyanobacterial cells, with less than 1% contamination by sponge cells. Gas chromatography/mass spectrometry analysis has revealed that the terpene spirodysin is concentrated in preparations containing archaeocytes and choanocytes, whereas nuclear magnetic resonance analysis of the symbiont cell preparations has shown that they usually contain the chlorinated diketopiperazines, dihydrodysamide C and didechlorodihydrodysamide C, which are the characteristic metabolites of the sponge/symbiont association. However, one symbiont preparation, partitioned by a second Percoll gradient, has been found to be devoid of chlorinated diketopiperazines. The capability to synthesize secondary metabolites may depend on the physiological state of the symbiont; alternatively, there may be two closely related cyanobacterial strains within the sponge tissue.

Key words Chlorinated alkaloids Percoll density gradient fractionation Secondary metabolites Cyanobacteria Oscillatoria spongeliae Sponges Dysidea herbacea (Porifera)

Copyright information

© Springer-Verlag Berlin Heidelberg 1998