Cell and Tissue Research

, Volume 289, Issue 3, pp 499–503

Immunohistochemical and immuno-electron-microscopic detection of interferon-γ-inducing factor (”interleukin-18”) in mouse intestinal epithelial cells

  • Makoto Takeuchi
  • Yasushi Nishizaki
  • Osamu Sano
  • Tsunetaka Ohta
  • Masao Ikeda
  • Masashi Kurimoto

DOI: 10.1007/s004410050895

Cite this article as:
Takeuchi, M., Nishizaki, Y., Sano, O. et al. Cell Tissue Res (1997) 289: 499. doi:10.1007/s004410050895

Abstract.

The novel cytokine interferon-γ-inducing factor (”interleukin-18”) is produced by macrophage-like cells in mice with endotoxin shock and induces the production of interferon-γ by T cells in vitro. To determine the physiological role for mouse interferon-γ-inducing factor, we studied its tissue distribution in several organs (intestine, spleen, thymus, kidney, and liver) in healthy mice of different ages, including fetal stages. Activity of the cytokine in the organ extracts of adult mice was measured by enzyme-linked immunosorbent assay, and the cellular distribution of interferon-γ-inducing factor in organs from fetal and adult mice was determined by immunohistochemistry. Intestinal extracts of adult mice showed the highest concentrations among the organs studied. Other organ extracts of adult mice showed lower concentrations of the cytokine. Immunohistochemical analysis revealed that interferon-γ-inducing factor was localized in the cytoplasm of intestinal epithelial cells from fetal and adult mice. These results show for the first time that intestinal epithelial cells may be the main producers of interferon-γ-inducing factor under normal physiological conditions and suggest that its constitutive expression in intestinal epithelial cells may have an important role in the induction of mucosal immunity.

Key words: Interferon-γ-inducing factor (”interleukin-18”)IntestineImmunohistochemistryMucosal immunityMouse (CD1)

Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • Makoto Takeuchi
    • 1
  • Yasushi Nishizaki
    • 1
  • Osamu Sano
    • 1
  • Tsunetaka Ohta
    • 1
  • Masao Ikeda
    • 1
  • Masashi Kurimoto
    • 1
  1. 1.Fujisaki Institute, Hayashibara Biochemical Laboratories, 675-1, Fujisaki, Okayama 702, JapanJP