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Isolation, characterization and immunomodulatory-associated gene transcription of Wharton’s jelly-derived multipotent mesenchymal stromal cells at different trimesters of cow pregnancy

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Abstract

The possibility of isolating bovine mesenchymal multipotent stromal cells (MSCs) from fetal adnexa is an interesting prospect due to the potential use of these cells in biotechnological applications. However, little is known about the properties of these progenitor cells in bovine species. Wharton’s jelly (WJ) MSC cells were obtained from the umbilical cord of bovine fetuses at three different stages of pregnancy and divided into groups 1, 2 and 3 according to gestational trimester. Cell morphology, from the three stages of pregnancy, typically appeared fibroblast-like spindle-shaped, presenting the same viability and number. Moreover, the proliferative ability of T-cells in response to a mitogenic stimulus was suppressed when WJMSC cells were added to the culture. Multilineage properties were confirmed by their ability to undergo adipogenic, osteogenic/chondrogenic and neurogenic differentiation. Mesenchymal phenotyping, CD105+, CD29+, CD73+ and CD90+ cell markers were detected in all three cell groups, yet these markers were considered more expressed in MSCs of group 2 (p < 0.005). Expression of cytokines IL2, IL6RR, INFAC, INFB1, IFNG, TNF and LTBR were downregulated, whereas IL1F10 expression was upregulated in all tested WJMSCs. The present study demonstrated that WJMSCs harvested from the bovine umbilical cord at different gestational stages showed proliferative capacity, immune privilege and stemness potential.

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Acknowledgments

The authors thank the cattle owners for their cooperation in the specimen collection at slaughterhouses. This work was supported by Fundação de Amparo à Pesquisa do Estado de São Paulo (Grants 2012/16715-4). T.C. Cardoso and E.F. Flores are recipients of CNPq (Brazilian Council for Research) funding.

Author’s contributions

TCC participated in the design of the study, performed the cell culture in all steps and flow cytometric analysis. LHO and JCB participated in the preparation of respective umbilical cords. RG and HLF participated also in the design of the study, performed the statistical analysis and drafted the manuscript. MM and EFF drafted the final version of the manuscript.

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Authors and Affiliations

  1. Laboratory of Animal Virology and Cell Culture College of Veterinary Medicine, UNESP- Sao Paulo State University, Araçatuba, São Paulo, 16050-680, Brazil

    Tereza C. Cardoso, Lucas H. Okamura, Jamila C. Baptistella, Roberto Gameiro & Márcia Marinho

  2. Domestic Animals Anatomy Section, College of Veterinary Medicine, UNESP- Sao Paulo State University, Araçatuba, São Paulo, 16050-680, Brazil

    Jamila C. Baptistella

  3. Domestic Animals Embryology Section, College of Veterinary Medicine, UNESP- Sao Paulo State University, Araçatuba, São Paulo, 16050-680, Brazil

    Roberto Gameiro

  4. Department of Veterinary Medicine, FZEA- USP- University of Sao Paulo, Av. Duque de Caxias Norte, 225, Pirassununga, SP, 13635-900, Brazil

    Helena L. Ferreira

  5. Departament of Preventive Veterinary Medicine, College of Veterinary Medicine, Federal University of Santa Maria, UFSM, Santa Maria, RS, 97115-900, Brazil

    Eduardo F. Flores

Authors
  1. Tereza C. Cardoso
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  2. Lucas H. Okamura
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  3. Jamila C. Baptistella
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  4. Roberto Gameiro
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  5. Helena L. Ferreira
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  6. Márcia Marinho
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  7. Eduardo F. Flores
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Correspondence to Tereza C. Cardoso.

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Cardoso, T.C., Okamura, L.H., Baptistella, J.C. et al. Isolation, characterization and immunomodulatory-associated gene transcription of Wharton’s jelly-derived multipotent mesenchymal stromal cells at different trimesters of cow pregnancy. Cell Tissue Res 367, 243–256 (2017). https://doi.org/10.1007/s00441-016-2504-9

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  • DOI: https://doi.org/10.1007/s00441-016-2504-9

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