Cell and Tissue Research

, Volume 360, Issue 1, pp 129–141

Light sheet-based fluorescence microscopy (LSFM) for the quantitative imaging of cells and tissues

  • Francesco Pampaloni
  • Bo-Jui Chang
  • Ernst H. K. Stelzer
Review

DOI: 10.1007/s00441-015-2144-5

Cite this article as:
Pampaloni, F., Chang, BJ. & Stelzer, E.H.K. Cell Tissue Res (2015) 360: 129. doi:10.1007/s00441-015-2144-5

Abstract

In light sheet-based fluorescence microscopy (LSFM), only the focal plane is illuminated by a laser light sheet. Hence, only the fluorophores within a thin volume of the specimen are excited. This reduces photo-bleaching and photo-toxic effects by several orders of magnitude compared with any other form of microscopy. Therefore, LSFM (aka single/selective-plane illumination microscopy [SPIM] or digitally scanned light sheet microscopy [DSLM]) is the technique of choice for the three-dimensional imaging of live or fixed and of small or large three-dimensional specimens. The parallel recording of millions of pixels with modern cameras provides an extremely fast acquisition speed. Recent developments address the penetration depth, the resolution and the recording speed of LSFM. The impact of LSFM on research areas such as three-dimensional cell cultures, neurosciences, plant biology and developmental biology is increasing at a rapid pace. The development of high-throughput LSFM is the next leap forward, allowing the application of LSFM in toxicology and drug discovery screening.

Keywords

Light sheet-based fluorescence microscopy (LSFM) Digitally scanned light sheet-based microscopy Single/selective-plane illumination microscopy Three-dimensional cell cultures Cellular spheroids High-throughput LSFM 

Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Francesco Pampaloni
    • 1
  • Bo-Jui Chang
    • 1
  • Ernst H. K. Stelzer
    • 1
  1. 1.Physical Biology Group (FB 15, IZN), Buchmann Institute for Molecular Life Sciences (BMLS, CEF-MC)Goethe Universität Frankfurt am Main (Campus Riedberg)Frankfurt am MainGermany