Abstract
We compared the bone regeneration potentials of autologous cultured osteoblasts and of bone-marrow-derived autologous MSCs in combination with allogeneic cancellous bone granules in a rabbit radial defect model. Radial shaft defects over 15 mm were made in 26 New Zealand white rabbits. The animals underwent insertion of allogeneic cancellous bone granules containing autologous osteoblasts into right-side defects (the experimental group) and of allogeneic cancellous bone granules with autologous MSCs into left-side defects (the control group). To quantitatively assess bone regeneration, radiographic evaluations as well as BMD and BMC measurements were performed 3, 6, 9 and 12 weeks post-implantation and histology as well as micro-CT image analysis were performed at 6 and 12 weeks. Radiographic evaluations 3 weeks post-implantation showed that the experimental group had a higher mean bone quantity index (p < 0.05) and micro-CT image analysis showed that experimental sides had a greater mean total regenerated bone volume and surface area than the control sides (p < 0.05). Histologic evaluations obtained at 6 and 12 weeks revealed distinctly greater granule resorption and new bone formation in the experimental group. This in vivo study demonstrates that a combination of autologous osteoblasts and small-sized, allogeneic cancellous bone granules leads to more rapid bone regeneration than autologous MSCs and small-sized, allogeneic cancellous bone granules.
References
Ahlmann E, Patzakis M, Roidis N (2002) Comparison of anterior and posterior iliac crest bone grafts in terms of harvest-site morbidity and functional outcomes. J Bone Joint Surg 84A:716–720
Arrington ED, Smith WJ, Chambers HG, Bucknell AL, Davino NA (1996) Complications of iliac crest bone graft harvesting. Clin Orthop Relat Res 329:300–309
Bruder SP, Fink DJ, Caplan AI (1994) Mesenchymal stem cells in bone development, bone repair, and skeletal regeneration therapy. J Cell Biochem 56:283–294
Caplan AI (1991) Mesenchymal stem cells. J Orthop Res 9:641–650
Friedenstein AJ, Piatetzky-Shapiro II, Petrakova KV (1996) Osteogenesis in transplants of bone marrow cells. J Embryol Exp Morphol 16:381–390
Gazdag AR, Lane JM, Glaser D, Foster RA (1995) Alternatives to autogenous bone graft: efficacy and indications. J Am Acad Orthop Surg 3:1–8
Herold HZ, Hurvitz A, Tadmor A (1971) The effect of growth hormone on the healing of experimental bone defects. Acta Orthop Scand 42:377–384
Kitoh H, Kitakoji T, Tsuchiya H, Mitsuyama H, Nakamura H, Katoh M, Ishiguro N (2004) Transplantation of marrow-derived mesenchymal stem cells and platelet-rich plasma during distraction osteogenesis—a preliminary result of three cases. Bone 35:892–898
Kon E, Muraglia A, Corsi A, Bianco P, Marcacci M, Martin I, Boyde A, Ruspantini I, Chistolini P, Rocca M, Giardino R, Cancedda R, Quarto R (2000) Autologous bone marrow stromal cells loaded onto porous hydroxyapatite ceramic accelerate bone repair in critical-size defects of sheep long bones. J Biomed Mater Res 49:328–337
Lee SU, Chung YG, Oh IH, Kim JM, Kim YS, Lee YG, Baek MH, Kim SE (2010) Bone regeneration using mesenchymal stem cells loaded onto allogeneic cancellous bone granules tissue engineering and regenerative medicine. Tissue Eng Regen Med 7:401–409
Parfitt AM, Drezner MK, Glorieux FH, Kanis JA, Malluche H, Meunier PJ, Ott SM, Recker RR (1987) Bone histomorphometry: standardization of nomenclature, symbols, and units. Report of the ASBMR Histomorphometry Nomenclature Committee. J Bone Miner Res 2:595–610
Petite H, Viateau V, Bensaid W, Meunier A, De Pollak C, Bourguignon M, Oudina K, Sedel L, Guillemin G (2000) Tissue-engineered bone regeneration. Nat Biotechnol 18:929–930
Pruss A, Perka C, Degenhardt P, Maronna U, Buttner-Janz K, Paul B, Müller K, Klumpp C, Bruck JC, Von Versen R (2002) Clinical efficacy and compatibility of allogeneic avital tissue transplants sterilized with a peracetic acid/ethanol mixture. Cell Tissue Bank 3:235–243
Schlegel KA, Donath K, Rupprecht S, Falk S, Zimmermann R, Felszeghy E, Wiltfang J (2004) De novo bone formation using bovine collagen and platelet-rich plasma. Biomaterials 25(23):5387–5393
Seebach C, Schultheiss J, Wilhelm K, Frannk J, Henrich D (2010) Comparison of six bone-graft substitutes regarding to cell seeding efficiency, metabolism and growth behaviour of human mesenchymal stem cells (MSC) in vitro. Injury 41:731–738
Steinwachs M (2009) New technique for cell-seeded collagen-matrix-supported autologous chondrocyte transplantation. Arthroscopy 25:208–211
Wiltfang J, Kloss FR, Kessler P, Nkenke E, Schultze-Mosgau S, Zimmermann R, Schlegel KA (2004) Effects of platelet-rich plasma on bone healing in combination with autogenous bone and bone substitutes in critical-size defects, An animal experiment. Clin Oral Implants Res 15:187–193
Yamanouchi K, Satomura K, Gotoh Y, Kitaoka E, Tobiume S, Kume K, Nagayama M (2001) Bone formation by transplanted human osteoblasts cultured within collagen sponge with dexamethasone in vitro. J Bone Miner Res 16:857–867
Zuk PA, Zhu M, Mizuno H, Huang J, Futrell JW, Katz AJ, Benhaim P, Lorenz HP, Hedrick MH (2001) Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Eng 7:211–228
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Kim, SJ., Chung, YG., Lee, YK. et al. Comparison of the osteogenic potentials of autologous cultured osteoblasts and mesenchymal stem cells loaded onto allogeneic cancellous bone granules. Cell Tissue Res 347, 303–310 (2012). https://doi.org/10.1007/s00441-011-1272-9
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DOI: https://doi.org/10.1007/s00441-011-1272-9