Cell and Tissue Research

, Volume 344, Issue 3, pp 471–479

Crohn’s disease: ultrastructure of interstitial cells in colonic myenteric plexus

  • Jüri J. Rumessen
  • Jean-Marie Vanderwinden
  • Thomas Horn
Regular Article

DOI: 10.1007/s00441-011-1175-9

Cite this article as:
Rumessen, J.J., Vanderwinden, J. & Horn, T. Cell Tissue Res (2011) 344: 471. doi:10.1007/s00441-011-1175-9

Abstract

The role of the interstitial cells of Cajal (ICC) in chronic inflammatory bowel disease, i.e., ulcerative colitis (UC) and Crohn’s disease (CD), remains unclear. Ultrastructural alterations in ICC in the colonic myenteric plexus (ICC-MP) have been reported previously in UC, but descriptions of ICC-MP and other interstitial cells in the myenteric region of the colon are lacking for CD. In the present study, we characterized the ultrastructure of interstitial cells, nerves, and glial cells in the myenteric region in Crohn’s colitis (CC). In comparison with controls, varicosities of the myenteric bundles were dilated and appeared to be empty. Lipid droplets and lipofuscin-bodies were prominent in glial cells and neurons. ICC-MP were scanty but, as in controls, had caveolae, prominent intermediate filaments, cytoplasmic dense bodies, and membrane-associated dense bands with a patchy basal lamina. ICC-MP were similar in the various colonic regions. ICC-MP in CC showed no signs of degeneration or cytological changes. As in controls, fibroblast-like cells had abundant coated vesicles but lacked prominent intermediate filaments and caveolae. Macrophages also appeared as in controls. In comparison with ICC-MP in UC, the cytology of ICC-MP in CC were thus undisturbed. The ultrastructural differences between UC and CC might reflect pathophysiological differences of importance for understanding pathogenetic differences between CD and UC.

Keywords

Interstitial cells of CajalCrohn’s colitisMyenteric plexusUltrastructureInflammatory bowel diseaseHuman

Abbreviations

CC

Crohn’s colitis

CD

Crohn’s disease

IBD

Inflammatory bowel disease

ICC

Interstitial cells of Cajal

ICC-MP

ICC associated with the myenteric plexus

ICC-SMP

ICC associated with the submuscular plexus

FLC

Fibroblast-like cells

gER

Granular endoplasmic reticulum

GI

Gastrointestinal

MP

Myenteric plexus

MC

Macrophages

PDGFRα

Platelet-derived growth factor receptor α

SK3

Small-conductance Ca++-activated K+ channels

UC

Ulcerative colitis

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Jüri J. Rumessen
    • 1
  • Jean-Marie Vanderwinden
    • 2
  • Thomas Horn
    • 3
  1. 1.Department of Gastroenterology F, Gentofte HospitalUniversity of CopenhagenHellerupDenmark
  2. 2.Laboratoire de Neurophysiologie, Faculté de MédecineUniversité Libre de BruxellesBrusselsBelgium
  3. 3.Department of PathologyUniversity Hospital Herlev and University of CopenhagenHerlevDenmark