Cell and Tissue Research

, Volume 341, Issue 3, pp 405–415

Ultrastructural evidence for human mast cell-eosinophil interactions in vitro

  • Yael Minai-Fleminger
  • Moran Elishmereni
  • Francesca Vita
  • Maria Rosa Soranzo
  • David Mankuta
  • Guliano Zabucchi
  • Francesca Levi-Schaffer
Regular Article

DOI: 10.1007/s00441-010-1010-8

Cite this article as:
Minai-Fleminger, Y., Elishmereni, M., Vita, F. et al. Cell Tissue Res (2010) 341: 405. doi:10.1007/s00441-010-1010-8

Abstract

We have hypothesized that mast cells (MC) and eosinophils (Eos), the main effectors of allergy, can form an effector unit. These cells co-exist in the inflamed tissues during the late and chronic stages of allergy and have been shown to be capable of influencing each other's survival and activity via soluble mediators. We have recently described couples of receptor-ligands that are expressed on either/both of these cells and that imply a physical interaction. In this study, we have investigated the existence of short-term (60 min) in vitro interactions between human peripheral blood Eos and cord-blood-derived MC by transmission electron microscopy. We have found that MC and Eos adhere to each other; the lipid body content and the granule morphology of co-cultured MC and Eos, respectively, are altered, and the level of Eos peroxidase (EPO) released by co-cultured Eos is elevated. Moreover, the transfer of EPO from Eos to MC and tryptase from MC to Eos has been observed. Our results thus indicate that, when co-cultured, MC and Eos show signs of physical contact and of reciprocal activation. This is the first in vitro evidence of functional physical interactions between human MC and Eos, interactions that might also occur in vivo during allergic diseases.

Keywords

Mast cellsEosinophilsEPOTryptaseElectron microscopyAllergyHuman

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Yael Minai-Fleminger
    • 1
  • Moran Elishmereni
    • 1
  • Francesca Vita
    • 3
  • Maria Rosa Soranzo
    • 3
    • 4
  • David Mankuta
    • 2
  • Guliano Zabucchi
    • 3
    • 4
  • Francesca Levi-Schaffer
    • 1
  1. 1.Pharmacology & Experimental Therapeutics Institute for Drug Research, School of Pharmacy, Faculty of MedicineThe Hebrew University of JerusalemJerusalemIsrael
  2. 2.Obstetrics and GynecologyHadassah University HospitalJerusalemIsrael
  3. 3.Physiology and PathologyUniversity of TriesteTriesteItaly
  4. 4.Life SciencesUniversity of TriesteTriesteItaly