Skip to main content

Advertisement

SpringerLink
Log in

Function analysis of mesenchymal Bcor in tooth development by using RNA interference

  • Regular Article
  • Published:
Cell and Tissue Research Aims and scope Submit manuscript

Abstract

Teeth, an excellent model for studying organogenesis, develop from a series of epithelial–mesenchymal interactions that are mediated by a complex molecular network. Bcor (BCL-6 interacting corepressor) has recently been discovered, but little is known about its function in tooth development. Mutations in BCOR affect humans with oculofaciocardiodental syndrome, which is an X-linked dominant disorder with presumed male lethality and which comprises microphthalmia, congenital cataracts, radiculomegaly, and cardiac and digital abnormalities. In this study, the Bcor expression pattern has been intensively investigated during mouse molar development. Bcor is expressed in both dental epithelium and the mesenchyme at E11.5. To understand the function of Bcor, knockdown of Bcor has been examined by using lentivirus-mediated RNA interference. Silencing of Bcor expression in dental mesenchymal cells at E14.5 causes dentinogenesis defects and retardation of tooth root development. Thus, our results suggest that Bcor expressed in the mesenchyme plays crucial roles during early tooth development. The function of Bcor expressed in the epithelium remains to be elucidated.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

We’re sorry, something doesn't seem to be working properly.

Please try refreshing the page. If that doesn't work, please contact support so we can address the problem.

References

  • Bajalica-Lagercrantz S, Piehl F, Farnebo F, Larsson C, Lagercrantz J (1998) Expression of the BCL6 gene in the pre- and postnatal mouse. Biochem Biophys Res Commun 247:357–360

    Article  CAS  PubMed  Google Scholar 

  • Cai J, Cho SW, Kim JY, Lee MJ, Cha YG, Jung HS (2007) Patterning the size and number of tooth and its cusps. Dev Biol 304:499–507

    Article  CAS  PubMed  Google Scholar 

  • Fan Z, Yamaza T, Lee JS, Yu J, Wang S, Fan G, Shi S, Wang CY (2009)BCOR regulates mesenchymal stem cell function by epigenetic mechanisms. Nat Cell Biol11:1002-1009

    Article  CAS  PubMed  Google Scholar 

  • Gearhart MD, Corcoran CM, Wamstad JA, Bardwell VJ (2006) Polycomb group and SCF ubiquitin ligases are found in a novel BCOR complex that is recruited to BCL6 targets. Mol Cell Biol 26:6880–6889

    Article  CAS  PubMed  Google Scholar 

  • Ghetu AF, Corcoran CM, Cerchietti L, Bardwell VJ, Melnick A, Privé GG (2008) Structure of a BCOR corepressor peptide in complex with the BCL6 BTB domain dimer. Mol Cell 29:384–391

    Article  CAS  PubMed  Google Scholar 

  • Hilton EN, Manson FD, Urquhart JE, Johnston JJ, Slavotinek AM, Hedera P, Stattin EL, Nordgren A, Biesecker LG, Black GC (2007) Left-sided embryonic expression of the BCL-6 corepressor, BCOR, is required for vertebrate laterality determination. Hum Mol Genet 16:1773–1782

    Article  CAS  PubMed  Google Scholar 

  • Huynh KD, Fischle W, Verdin E, Bardwell VJ (2000) BCoR, a novel corepressor involved in BCL-6 repression. Genes Dev 14:1810–1823

    CAS  PubMed  Google Scholar 

  • Jardin F, Ruminy P, Bastard C, Tilly H (2007) The BCL6 proto-oncogene: a leading role during germinal center development and lymphomagenesis. Pathol Biol (Paris) 55:73–83

    CAS  Google Scholar 

  • Jernvall J, Thesleff I (2000) Reiterative signaling and patterning during mammalian tooth morphogenesis. Mech Dev 92:19–29

    Article  CAS  PubMed  Google Scholar 

  • Kim JW, Simmer JP (2007) Hereditary dentin defects. J Dent Res 86:392–399

    Article  CAS  PubMed  Google Scholar 

  • Lee JM, Kim JY, Cho KW, Lee MJ, Cho SW, Kwak S, Cai J, Jung HS (2008)Wnt11/Fgfr1b cross-talk modulates the fate of cells in palate development.Dev Biol 314:341–350

    CAS  PubMed  Google Scholar 

  • Lumsden AG (1988) Spatial organization of the epithelium and the role of neural crest cells in the initiation of the mammalian tooth germ. Development 103:155–169

    PubMed  Google Scholar 

  • Mina M, Kollar EJ (1987) The induction of odontogenesis in non-dental mesenchyme combined with early murine mandibular arch epithelium. Arch Oral Biol 32:123–127

    Article  CAS  PubMed  Google Scholar 

  • Ng D, Thakker N, Corcoran CM, Donnai D, Perveen R, Schneider A, Hadley DW, Tifft C, Zhang L, Wilkie AO, Smagt JJ van der, Gorlin RJ, Burgess SM, Bardwell VJ, Black GC, Biesecker LG (2004) Oculofaciocardiodental and Lenz microphthalmia syndromes result from distinct classes of mutations in BCOR. Nat Genet 36:411–416

    Article  CAS  PubMed  Google Scholar 

  • Oberoi S, Winder AE, Johnston J, Vargervik K, Slavotinek AM (2005) Case reports of oculofaciocardiodental syndrome with unusual dental findings. Am J Med Genet A 136:275–277

    PubMed  Google Scholar 

  • Ogawa T, Onishi T, Hayashibara T, Sakashita S, Okawa R, Ohshima T (2006) Dentinal defects in Hyp mice not caused by hypophosphatemia alone. Arch Oral Biol 51:58–63

    Article  CAS  PubMed  Google Scholar 

  • Pagan JK, Arnold J, Hanchard KJ, Kumar R, Bruno T, Jones MJ, Richard DJ, Forrest A, Spurdle A, Verdin E, Crossley M, Fanciulli M, Chenevix-Trench G, Young DB, Khanna KK (2007) A novel corepressor, BCoR-L1, represses transcription through an interaction with CtBP. J Biol Chem 282:15248–15257

    Article  CAS  PubMed  Google Scholar 

  • Pispa J, Thesleff I (2003) Mechanisms of ectodermal organogenesis. Dev Biol 262:195–205

    Article  CAS  PubMed  Google Scholar 

  • Plikus MV, Zeichner-David M, Mayer JA, Reyna J, Bringas P, Thewissen JG, Snead ML, Chai Y, Chuong CM (2005) Morphoregulation of teeth: modulating the number, size, shape and differentiation by tuning Bmp activity. Evol Dev 7:440–457

    Article  CAS  PubMed  Google Scholar 

  • Song Y, Zhang Z, Yu X, Yan M, Zhang X, Gu S, Stuart T, Liu C, Reiser J, Zhang Y, Chen Y (2006) Application of lentivirus-mediated RNAi in studying gene function in mammalian tooth development. Dev Dyn 235:1334–1344

    Article  CAS  PubMed  Google Scholar 

  • Ten Cate AR (1998) Oral histology: development, structure, and function, 5th edn, Mosby-Year Book, St. Louis

    Google Scholar 

  • Wamstad JA, Bardwell VJ (2007) Characterization of Bcor expression in mouse development. Gene Expr Patterns 7:550–557

    Article  CAS  PubMed  Google Scholar 

  • Yoshikawa DK, Kollar EJ (1981) Recombination experiments on the odontogenic roles of mouse dental papilla and dental sac tissues in ocular grafts. Arch Oral Biol 26:303–307

    Article  CAS  PubMed  Google Scholar 

  • Zhang Z, Song Y, Zhang X, Tang J, Chen J, Chen Y (2003) Msx1/Bmp4 genetic pathway regulates mammalian alveolar bone formation via induction of Dlx5 and Cbfa1. Mech Dev 120:1469–1479

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

The authors thank Prof. Yiping Chen for providing EGFP-, Msx1i-expressing lentiviruses and Prof. Jakob Reiser for providing pNL-CMV vector, helper plasmid pCD-NL/BH*ΔΔΔ, and envelope plasmid pLTR-G.

Author information

Authors and Affiliations

  1. Division in Anatomy and Developmental Biology, Department of Oral Biology, Research Center for Orofacial Hard Tissue Regeneration, Brain Korea 21 Project, Oral Science Research Center, College of Dentistry, Yonsei Center of Biotechnology, Yonsei University, Seoul, South Korea

    Jinglei Cai, Sungwook Kwak, Jong-Min Lee, Eun-Jung Kim, Min-Jung Lee, Gi-Hee Park, Sung-Won Cho & Han-Sung Jung

  2. Key Laboratory of Regenerative Biology,South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, 510530, Guangzhou, China

    Jinglei Cai

Authors
  1. Jinglei Cai
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Sungwook Kwak
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jong-Min Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Eun-Jung Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Min-Jung Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Gi-Hee Park
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Sung-Won Cho
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Han-Sung Jung
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Han-Sung Jung.

Additional information

This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science, and Technology (R13-2003-013-05001-0).

Jinglei Cai and Sungwook Kwak contributed equally to this work.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Cai, J., Kwak, S., Lee, JM. et al. Function analysis of mesenchymal Bcor in tooth development by using RNA interference. Cell Tissue Res 341, 251–258 (2010). https://doi.org/10.1007/s00441-010-0996-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00441-010-0996-2

Keywords

Search

Navigation