Cell and Tissue Research

, Volume 339, Issue 3, pp 649–653

Pituitary adenylate cyclase-activating polypeptide type 1 (PAC1) receptor is expressed during embryonic development of the earthworm

  • Ákos Boros
  • Ildikó Somogyi
  • Péter Engelmann
  • Andrea Lubics
  • Dóra Reglodi
  • Edit Pollák
  • László Molnár
Short Communication

DOI: 10.1007/s00441-009-0909-4

Cite this article as:
Boros, Á., Somogyi, I., Engelmann, P. et al. Cell Tissue Res (2010) 339: 649. doi:10.1007/s00441-009-0909-4

Abstract

Pituitary adenylate cyclase activating polypeptide (PACAP)-like molecules have been shown to be present in cocoon albumin and in Eisenia fetida embryos at an early developmental stage (E1) by immunocytochemistry and radioimmunoassay. Here, we focus on detecting the stage at which PAC1 receptor (PAC1R)-like immunoreactivity first appears in germinal layers and structures, e.g., various parts of the central nervous system (CNS), in developing earthworm embryos. PAC1R-like immunoreactivity was revealed by Western blot and Far Western blot as early as the E2 developmental stage, occurring in the ectoderm and later in specific neurons of the developing CNS. Labeled CNS neurons were first seen in the supraesophageal ganglion (brain) and subsequently in the subesophageal and ventral nerve cord ganglia. Ultrastructurally, PAC1Rs were located mainly on plasma membranes and intracellular membranes, especially on cisternae of the endoplasmic reticulum. Therefore, PACAP-like compounds probably influence the differentiation of germinal layers (at least the ectoderm) and of some neurons and might act as signaling molecules during earthworm embryonic development.

Keywords

Pituitary adenylate cyclase activating polypeptideWestern blotFar Western blotImmunocytochemistryEisenia fetida (Annelida)

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Ákos Boros
    • 1
  • Ildikó Somogyi
    • 1
  • Péter Engelmann
    • 2
  • Andrea Lubics
    • 3
  • Dóra Reglodi
    • 3
    • 4
  • Edit Pollák
    • 1
  • László Molnár
    • 1
  1. 1.Department of General Zoology, Faculty of SciencesUniversity of PécsPécsHungary
  2. 2.Department of Immunology and Biotechnology, Clinical CenterUniversity of PécsPécsHungary
  3. 3.Department of Anatomy, Medical SchoolUniversity of PécsPécsHungary
  4. 4.Department of AnatomyRoss University Medical SchoolPortsmouthWest Indies