Cell and Tissue Research

, Volume 339, Issue 3, pp 649–653

Pituitary adenylate cyclase-activating polypeptide type 1 (PAC1) receptor is expressed during embryonic development of the earthworm

Authors

  • Ákos Boros
    • Department of General Zoology, Faculty of SciencesUniversity of Pécs
  • Ildikó Somogyi
    • Department of General Zoology, Faculty of SciencesUniversity of Pécs
  • Péter Engelmann
    • Department of Immunology and Biotechnology, Clinical CenterUniversity of Pécs
  • Andrea Lubics
    • Department of Anatomy, Medical SchoolUniversity of Pécs
  • Dóra Reglodi
    • Department of Anatomy, Medical SchoolUniversity of Pécs
    • Department of AnatomyRoss University Medical School
  • Edit Pollák
    • Department of General Zoology, Faculty of SciencesUniversity of Pécs
    • Department of General Zoology, Faculty of SciencesUniversity of Pécs
Short Communication

DOI: 10.1007/s00441-009-0909-4

Cite this article as:
Boros, Á., Somogyi, I., Engelmann, P. et al. Cell Tissue Res (2010) 339: 649. doi:10.1007/s00441-009-0909-4

Abstract

Pituitary adenylate cyclase activating polypeptide (PACAP)-like molecules have been shown to be present in cocoon albumin and in Eisenia fetida embryos at an early developmental stage (E1) by immunocytochemistry and radioimmunoassay. Here, we focus on detecting the stage at which PAC1 receptor (PAC1R)-like immunoreactivity first appears in germinal layers and structures, e.g., various parts of the central nervous system (CNS), in developing earthworm embryos. PAC1R-like immunoreactivity was revealed by Western blot and Far Western blot as early as the E2 developmental stage, occurring in the ectoderm and later in specific neurons of the developing CNS. Labeled CNS neurons were first seen in the supraesophageal ganglion (brain) and subsequently in the subesophageal and ventral nerve cord ganglia. Ultrastructurally, PAC1Rs were located mainly on plasma membranes and intracellular membranes, especially on cisternae of the endoplasmic reticulum. Therefore, PACAP-like compounds probably influence the differentiation of germinal layers (at least the ectoderm) and of some neurons and might act as signaling molecules during earthworm embryonic development.

Keywords

Pituitary adenylate cyclase activating polypeptideWestern blotFar Western blotImmunocytochemistryEisenia fetida (Annelida)

Copyright information

© Springer-Verlag 2010