Cell and Tissue Research

, 338:401

Isolation, characterization and osteogenic differentiation of adipose-derived stem cells: from small to large animal models

  • Elena Arrigoni
  • Silvia Lopa
  • Laura de Girolamo
  • Deborah Stanco
  • Anna T. Brini
Regular Article

DOI: 10.1007/s00441-009-0883-x

Cite this article as:
Arrigoni, E., Lopa, S., de Girolamo, L. et al. Cell Tissue Res (2009) 338: 401. doi:10.1007/s00441-009-0883-x

Abstract

One of the most important issues in orthopaedic surgery is the loss of bone resulting from trauma, infections, tumours or congenital deficiency. In view of the hypothetical future application of mesenchymal stem cells isolated from human adipose tissue in regenerative medicine, we have analysed and characterized adipose-derived stem cells (ASCs) isolated from adipose tissue of rat, rabbit and pig. We have compared their in vitro osteogenic differentiation abilities for exploitation in the repair of critical osteochondral defects in autologous pre-clinical models. The number of pluripotent cells per millilitre of adipose tissue is variable and the yield of rabbit ASCs is lower than that in rat and pig. However, all ASCs populations show both a stable doubling time during culture and a marked clonogenic ability. After exposure to osteogenic stimuli, ASCs from rat, rabbit and pig exhibit a significant increase in the expression of osteogenic markers such as alkaline phosphatase, extracellular calcium deposition, osteocalcin and osteonectin. However, differences have been observed depending on the animal species and/or differentiation period. Rabbit and porcine ASCs have been differentiated on granules of clinical grade hydroxyapatite (HA) towards osteoblast-like cells. These cells grow and adhere to the scaffold, with no inhibitory effect of HA during osteo-differentiation. Such in vitro studies are necessary in order to select suitable pre-clinical models to validate the use of autologous ASCs, alone or in association with proper biomaterials, for the repair of critical bone defects.

Keywords

Adipose-derived stem cells Osteogenic differentiation Biomaterials Hydroxyapatite Tissue engineering Rat Pig Rabbit 

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Elena Arrigoni
    • 1
  • Silvia Lopa
    • 1
  • Laura de Girolamo
    • 2
  • Deborah Stanco
    • 1
  • Anna T. Brini
    • 1
  1. 1.Department of Medical Pharmacology, Faculty of Medicine Università degli Studi di MilanoMilanItaly
  2. 2.IRCCS Galeazzi Orthopaedic InstituteMilanItaly

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