Cell and Tissue Research

, Volume 338, Issue 2, pp 283–293

Thymic stromal lymphopoietin enhances tight-junction barrier function of human nasal epithelial cells

  • Ryuta Kamekura
  • Takashi Kojima
  • Jun-ichi Koizumi
  • Noriko Ogasawara
  • Makoto Kurose
  • Mitsuru Go
  • Atsushi Harimaya
  • Masaki Murata
  • Satoshi Tanaka
  • Hideki Chiba
  • Tetsuo Himi
  • Norimasa Sawada
Regular Article

DOI: 10.1007/s00441-009-0855-1

Cite this article as:
Kamekura, R., Kojima, T., Koizumi, J. et al. Cell Tissue Res (2009) 338: 283. doi:10.1007/s00441-009-0855-1
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Abstract

Epithelial-derived thymic stromal lymphopoietin (TSLP) triggers dendritic cell (DC)-mediated Th2-type inflammatory responses and is a master switch for allergic inflammatory diseases. In the present study, the expression and induction of TSLP and the effects of TSLP on the tight-junctional barrier of human nasal epithelial cells (HNECs) have been investigated in order to elucidate the role of TSLP in allergic rhinitis. We have found high expression of TSLP in the epithelium from patients with allergic rhinitis with recruitment and infiltration of DCs. In vitro, TSLP is significantly produced in HNECs after treatment with a toll-like receptor 2 (TLR2) ligand, Pam3Cys-Ser-(Lys)4, and a mixture of interleukin-1β and tumor necrosis factor-α. Treatment with TSLP rapidly enhances the barrier function of cultured HNECs, together with an increase of tight-junction proteins claudin-1, -4, -7, and occludin. The nasal-epithelial-derived TSLP thus not only activates DCs but also preserves the epithelial barrier via the upregulation of tight-junction proteins, thereby regulating antigen sensitization during the early stage of allergic rhinitis.

Keywords

Thymic stromal lymphopoietin Tight junctions Barrier function Nasal epithelial cells Allergic rhinitis Human 

Abbreviations

DAPI

4′,6-diamidino-2-phenylindole dihydrochloride

DC

dendritic cell

HNEC

human nasal epithelial cell

IL

interleukin

JAM

junctional adhesion molecule

LCA

leukocyte common antigen

LPS

lipopolysaccharide

P3CSK4

Pam3Cys-Ser-(Lys)4

RT-PCR

reverse transcription with polymerase chain reaction

Poly (I:C)

polyinosine-polycytidylic acid

TER

transepithelial electrical resistance

TGF-β

transforming growth factor-β

TNF

tumor necrosis factor

TSLP

thymic stromal lymphopoietin

TSLPR

part of the functional TSLP receptor

ZO

zonula occludens

Supplementary material

441_2009_855_Fig1_ESM.gif (300 kb)
Fig. S1: Supplemental data 1

Immunocytochemistry for tight-junction proteins claudin-1, -4, and -7 in cultured HNECs after treatment with TSLP. Bars 20 μm

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High resolution image file (TIFF 2885 kb)
441_2009_855_Fig2_ESM.gif (43 kb)
Fig. S2: Supplemental data 2

Western blotting for tight-junction proteins in normal nasal mucosa and in that from patients with allergic rhinitis (CL claudin). The corresponding expression levels are shown as bar graphs. Error bars represent means±SE; n=3, **P<0.01 versus normal mucosa

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High resolution image file (TIFF 1589 kb)
441_2009_855_Fig3_ESM.gif (17 kb)
Fig. S3: Supplemental data 3

RT-PCR for TSLPR and IL-7Rα in human nasal mucosa (in vivo) and cultured HNECs (in vitro); M 100-bp ladder DNA marker

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High resolution image file (TIFF 1059 kb)

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Ryuta Kamekura
    • 1
    • 2
  • Takashi Kojima
    • 2
  • Jun-ichi Koizumi
    • 1
  • Noriko Ogasawara
    • 1
    • 2
  • Makoto Kurose
    • 1
  • Mitsuru Go
    • 1
  • Atsushi Harimaya
    • 1
  • Masaki Murata
    • 2
  • Satoshi Tanaka
    • 2
  • Hideki Chiba
    • 2
  • Tetsuo Himi
    • 1
  • Norimasa Sawada
    • 2
  1. 1.Department of OtolaryngologySapporo Medical University School of MedicineSapporoJapan
  2. 2.Department of PathologySapporo Medical University School of MedicineSapporoJapan

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