Regular Article

Cell and Tissue Research

, Volume 338, Issue 2, pp 283-293

Thymic stromal lymphopoietin enhances tight-junction barrier function of human nasal epithelial cells

  • Ryuta KamekuraAffiliated withDepartment of Otolaryngology, Sapporo Medical University School of MedicineDepartment of Pathology, Sapporo Medical University School of Medicine
  • , Takashi KojimaAffiliated withDepartment of Pathology, Sapporo Medical University School of Medicine Email author 
  • , Jun-ichi KoizumiAffiliated withDepartment of Otolaryngology, Sapporo Medical University School of Medicine
  • , Noriko OgasawaraAffiliated withDepartment of Otolaryngology, Sapporo Medical University School of MedicineDepartment of Pathology, Sapporo Medical University School of Medicine
  • , Makoto KuroseAffiliated withDepartment of Otolaryngology, Sapporo Medical University School of Medicine
  • , Mitsuru GoAffiliated withDepartment of Otolaryngology, Sapporo Medical University School of Medicine
  • , Atsushi HarimayaAffiliated withDepartment of Otolaryngology, Sapporo Medical University School of Medicine
  • , Masaki MurataAffiliated withDepartment of Pathology, Sapporo Medical University School of Medicine
  • , Satoshi TanakaAffiliated withDepartment of Pathology, Sapporo Medical University School of Medicine
    • , Hideki ChibaAffiliated withDepartment of Pathology, Sapporo Medical University School of Medicine
    • , Tetsuo HimiAffiliated withDepartment of Otolaryngology, Sapporo Medical University School of Medicine
    • , Norimasa SawadaAffiliated withDepartment of Pathology, Sapporo Medical University School of Medicine

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access

Abstract

Epithelial-derived thymic stromal lymphopoietin (TSLP) triggers dendritic cell (DC)-mediated Th2-type inflammatory responses and is a master switch for allergic inflammatory diseases. In the present study, the expression and induction of TSLP and the effects of TSLP on the tight-junctional barrier of human nasal epithelial cells (HNECs) have been investigated in order to elucidate the role of TSLP in allergic rhinitis. We have found high expression of TSLP in the epithelium from patients with allergic rhinitis with recruitment and infiltration of DCs. In vitro, TSLP is significantly produced in HNECs after treatment with a toll-like receptor 2 (TLR2) ligand, Pam3Cys-Ser-(Lys)4, and a mixture of interleukin-1β and tumor necrosis factor-α. Treatment with TSLP rapidly enhances the barrier function of cultured HNECs, together with an increase of tight-junction proteins claudin-1, -4, -7, and occludin. The nasal-epithelial-derived TSLP thus not only activates DCs but also preserves the epithelial barrier via the upregulation of tight-junction proteins, thereby regulating antigen sensitization during the early stage of allergic rhinitis.

Keywords

Thymic stromal lymphopoietin Tight junctions Barrier function Nasal epithelial cells Allergic rhinitis Human