Cell and Tissue Research

, Volume 336, Issue 3, pp 521–527

Nuclear transport of protein TTC4 depends on the cell cycle

  • Ruslan I. Dmitriev
  • Irina A. Okkelman
  • Roman A. Abdulin
  • Mikhail I. Shakhparonov
  • Nikolay B. Pestov
Short Communication

DOI: 10.1007/s00441-009-0785-y

Cite this article as:
Dmitriev, R.I., Okkelman, I.A., Abdulin, R.A. et al. Cell Tissue Res (2009) 336: 521. doi:10.1007/s00441-009-0785-y

Abstract

TTC4 (tetratricopeptide repeat domain protein 4) is a putative tumor suppressor involved in the transformation of melanocytes. At present, the relationships between TTC4 and DNA replication proteins are largely unknown, as are the tissue distribution and subcellular localization of TTC4. Using reverse transcription with the polymerase chain reaction, we have observed that the murine TTC4 gene is ubiquitously expressed. Analysis of the TTC4 subcellular localization has shown that, upon overexpression, TTC4 localizes to the cytoplasm. Interestingly, co-expression with a known protein interaction partner, hampin/MSL1, results in the nuclear translocation of the TTC4 protein. The subcellular localization of endogenous TTC4 depends, however, on the cell cycle: it is mostly nuclear in the G1 and S phases and is evenly distributed between the nucleus and cytoplasm in G2. The nuclear transport of TTC4 is apparently a complex process dependent on interactions with other proteins during the progression of the cell cycle. Thus, the dynamic character of the nuclear accumulation of TTC4 might be a potential link with regard to its function in tumor suppression.

Keywords

TTC4HampinCell cycleMouseTPR

Supplementary material

441_2009_785_Fig1_ESM.gif (201 kb)
Figure S1

Heterogeneity of subcellular location of endogenous TTC4 protein in asynchronous fibroblasts. Sections 1 and 3 show a typical cell with predominantly nuclear TTC4, whereas in sections 2, 4 another cell from the same culture has TTC4 evenly distributed between its nucleus and cytoplasm. 3T3 cells were fixed in cold methanol and stained with anti-TTC4 antibodies followed by Alexa Fluor 488-conjugated anti-rabbit antibodies (green fluorescence) and counterstained with DAPI (blue fluorescence). 1,2 - green channel, 3,4 - merge. Bar, 50 μm. (GIF 200 KB)

441_2009_785_Fig1_ESM.tif (5.5 mb)
High resolution image file (TIF 8.3 MB)
441_2009_785_Fig2_ESM.gif (75 kb)
Figure S1

Heterogeneity of subcellular location of endogenous TTC4 protein in asynchronous fibroblasts. Sections 1 and 3 show a typical cell with predominantly nuclear TTC4, whereas in sections 2, 4 another cell from the same culture has TTC4 evenly distributed between its nucleus and cytoplasm. 3T3 cells were fixed in cold methanol and stained with anti-TTC4 antibodies followed by Alexa Fluor 488-conjugated anti-rabbit antibodies (green fluorescence) and counterstained with DAPI (blue fluorescence). 1,2 - green channel, 3,4 - merge. Bar, 50 μm. (GIF 200 KB)

441_2009_785_Fig2_ESM.tif (19.9 mb)
High resolution image file (TIF 19.8 MB)
441_2009_785_Fig3_ESM.gif (72 kb)
Figure S1

Heterogeneity of subcellular location of endogenous TTC4 protein in asynchronous fibroblasts. Sections 1 and 3 show a typical cell with predominantly nuclear TTC4, whereas in sections 2, 4 another cell from the same culture has TTC4 evenly distributed between its nucleus and cytoplasm. 3T3 cells were fixed in cold methanol and stained with anti-TTC4 antibodies followed by Alexa Fluor 488-conjugated anti-rabbit antibodies (green fluorescence) and counterstained with DAPI (blue fluorescence). 1,2 - green channel, 3,4 - merge. Bar, 50 μm. (GIF 200 KB)

441_2009_785_Fig3_ESM.tif (7.2 mb)
High resolution image file (TIF 12.8 MB)

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Ruslan I. Dmitriev
    • 1
  • Irina A. Okkelman
    • 1
  • Roman A. Abdulin
    • 1
  • Mikhail I. Shakhparonov
    • 1
  • Nikolay B. Pestov
    • 1
  1. 1.Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of SciencesMoscowRussia