Cell and Tissue Research

, Volume 335, Issue 3, pp 505–515

Impact of Sur1 gene inactivation on the morphology of mouse pancreatic endocrine tissue

  • Ihsane Marhfour
  • Pierre Moulin
  • Joëlle Marchandise
  • Jacques Rahier
  • Christine Sempoux
  • Yves Guiot
Regular Article

DOI: 10.1007/s00441-008-0733-2

Cite this article as:
Marhfour, I., Moulin, P., Marchandise, J. et al. Cell Tissue Res (2009) 335: 505. doi:10.1007/s00441-008-0733-2

Abstract

In congenital hyperinsulinism of infancy (CHI), the loss of K-ATP channels (composed of Kir6.2 and SUR1 subunits) in β cells induces permanent insulin secretion and severe hypoglycaemia. By contrast, Sur1−/− mice do not present such defects. We have investigated the impact of Sur1 gene inactivation on mouse islet cell morphology, structure and basic physiology. Pancreata were collected from young, adult and old wild-type (WT) and Sur1−/− mice. After immunostaining for hormone, the total endocrine tissue, cell proportion, cell size and intra-insular distribution, hormone content and Glut-2 expression were quantified by morphometry. Basic physiological parameters were also measured. In young Sur1−/− mice, the total endocrine tissue and proportion of β cells were higher (P<0.05) than in WT mice, whereas the proportion of δ cells was lower (P<0.01). In old Sur1−/− mice, α cells were frequently located in the central regions of islets (unlike WT islets) and their proportion was increased (P<0.05). Glut-2 protein and mRNA levels were lower in old Sur1−/− islets (P<0.02). Insulinaemia, fasting insulin and glucagon contents were equivalent in both groups of pancreata. Thus, the islets of Sur1−/− mice present morphological modifications that have not been described in CHI and that might reflect an adaptive mechanism controlling insulin secretion in these mice.

Keywords

Congenital hyperinsulinism Endocrine pancreatic cells K-ATP channel Morphology Sur1 knockout mice 

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Ihsane Marhfour
    • 1
  • Pierre Moulin
    • 1
  • Joëlle Marchandise
    • 1
  • Jacques Rahier
    • 1
  • Christine Sempoux
    • 1
  • Yves Guiot
    • 1
    • 2
  1. 1.Department of Pathology, Faculty of MedicineUniversité Catholique de LouvainBrusselsBelgium
  2. 2.Department of PathologyCliniques Universitaires St-LucBrusselsBelgium

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