Cell and Tissue Research

, Volume 328, Issue 3, pp 537–548

Elevated expression of genes assigned to NF-κB and apoptotic pathways in human periodontal ligament fibroblasts following mechanical stretch

  • Nina Ritter
  • Eva Mussig
  • Thorsten Steinberg
  • Annette Kohl
  • Gerda Komposch
  • Pascal Tomakidi
Regular Article

DOI: 10.1007/s00441-007-0382-x

Cite this article as:
Ritter, N., Mussig, E., Steinberg, T. et al. Cell Tissue Res (2007) 328: 537. doi:10.1007/s00441-007-0382-x
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Abstract

There is growing evidence that apoptosis involves the nuclear transcription factor NF-κB in conjunction with related genes. However, in the context of mechanical orthodontic forces, force-sensing target genes assigned to pathways of NF-κB and apoptosis have not been fully characterised. To contribute to the identification of putative target genes, we used cDNA arrays specific for NF-κB and apoptotic pathways and analysed elevated gene expression in primary human periodontal ligament fibroblasts (PDL-F) after a 6 h application of mechanical force. Among several identified genes (including several caspases), interleukin-1β (IL-1β) and NF-κB displayed significantly higher expression on the NF-κB array, whereas higher expression was obtained for BCL2-antagonist of cell death (BAD), member 6 of the TNF-receptor superfamily (FAS) and CASP2 and RIPK1 domain-containing adaptor with death domain (CRADD) on the apoptosis array. Based on a defined cut-off level of a more than 1.5-fold higher expression, this significance in elevated gene expression was corroborated by reverse transcription/polymerase chain reaction (RT-PCR). Here, semi-quantitative (sq) PCR revealed a more pronounced elevation of mRNA gene expression in PDL-F after 6 h of stretch, when compared with 12 h. Moreover, the elevation after 6 h as observed by sq-PCR was convergent with quantitative PCR (q-PCR). q-PCR yielded levels of 5.8-fold higher relative gene expression for IL-1β and 1.7-fold for NF-κB, whereas that computed for BAD indicated a 5.2-fold, for CRADD a 2.1-fold and for FAS a 2.0-fold higher expression. The data obtained from the expression analysis thus indicate a stretch-induced transcriptional elevation of genes assigned to the NF-κB and apoptotic pathways. This elevation may render them target candidates for being addressed by mechanical orthodontic forces.

Keywords

Periodontal ligament Periodontal ligament fibroblasts Mechanical stretch Apoptosis cDNA microarray Human 

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Nina Ritter
    • 1
  • Eva Mussig
    • 1
  • Thorsten Steinberg
    • 1
  • Annette Kohl
    • 1
  • Gerda Komposch
    • 1
  • Pascal Tomakidi
    • 1
  1. 1.Department of Orthodontics and Dentofacial Orthopaedics, Dental SchoolUniversity of HeidelbergHeidelbergGermany