Cell and Tissue Research

, Volume 328, Issue 3, pp 515–520

In-vivo intratissue ablation by nanojoule near-infrared femtosecond laser pulses

  • Bao-Gui Wang
  • Iris Riemann
  • Harald Schubert
  • Karl-Juergen Halbhuber
  • Karsten Koenig
Regular Article

DOI: 10.1007/s00441-006-0367-1

Cite this article as:
Wang, BG., Riemann, I., Schubert, H. et al. Cell Tissue Res (2007) 328: 515. doi:10.1007/s00441-006-0367-1

Abstract

Non-invasive intratissue ablation was performed in the cornea of living rabbits by using 80 MHz near-infrared intense nanojoule femtosecond laser pulses. The intratissue surgical effect was induced by multiphoton absorption at a wavelength of 800 nm and was ascertained by histological examination. Highly precise intratissue ablation was obtained with no detrimental effects to the overlying or underlying layers. Activated keratocytes in the laser-treated corneas were detected with two-photon imaging postoperatively. Intratissue femtosecond laser ablation thus has potential as a effective technique in refractive surgery for the treatment of visual disorders.

Keywords

AblationEye surgeryMyofibroblastsFemtosecond laserNanoprocessingRabbit (New Zealand Albino)

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Bao-Gui Wang
    • 1
  • Iris Riemann
    • 2
  • Harald Schubert
    • 3
  • Karl-Juergen Halbhuber
    • 1
  • Karsten Koenig
    • 2
    • 4
    • 5
  1. 1.Institute of Microscopic Anatomy (Anatomy II)University of JenaJenaGermany
  2. 2.Fraunhofer Institute of Biomedical Technology (IBMT)St. IngbertGermany
  3. 3.Institute of Laboratory Animals ScienceUniversity of JenaJenaGermany
  4. 4.Faculty of Physics and MechatronicsSaarland UniversitySaarbrückenGermany
  5. 5.JenLabJenaGermany