Regular Article

Cell and Tissue Research

, Volume 327, Issue 1, pp 25-31

Expression of UNCL during development of periodontal tissue and response of periodontal ligament fibroblasts to mechanical stress in vivo and in vitro

  • Heung-Joong KimAffiliated withOral Biology Research Institute, College of Dentistry, Chosun University
  • , Yong Seok ChoiAffiliated withDepartment of Oral Histology, College of Dentistry, Chosun University
  • , Moon-Jin JeongAffiliated withDepartment of Oral Histology, College of Dentistry, Chosun University
  • , Byung-Ock KimAffiliated withOral Biology Research Institute, College of Dentistry, Chosun University
  • , Sung-Hoon LimAffiliated withOral Biology Research Institute, College of Dentistry, Chosun University
  • , Do Kyung KimAffiliated withOral Biology Research Institute, College of Dentistry, Chosun University
  • , Chong Kwan KimAffiliated withResearch Institute of Periodontal Regeneration, Yonsei University
  • , Joo-Cheol ParkAffiliated withDepartment of Oral Histology, College of Dentistry, Chosun University Email author 

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Abstract

Mutations in two genes, uncoordinated (unc) and uncoordinated-like (uncl), lead to a failure of mechanotransduction in Drosophila. UNCL, the human homolog of unc and uncl, is preferentially expressed in periodontal ligament (PDL) fibroblasts compared with gingival fibroblasts. However, the precise role of UNCL in the PDL remains unclear. The aim of the present study has been to examine whether mechanical stimuli modulate the expression of UNCL in the human PDL in vivo and in vitro and to examine the roles of UNCL in the development, regeneration, and repair of the PDL. We have investigated the expression pattern of UNCL during the development of periodontal tissue and the response of PDL fibroblasts to mechanical stress in vivo and in vitro. The expression of UNCL mRNA and protein increases with PDL fibroblast differentiation from the confluent to multilayer stage but slightly decreases on mineralized nodule formation. UNCL has also been localized in ameloblasts and adjacent cells, differentiating cementoblasts, and osteoblasts of the developing tooth. Strong distinct UNCL expression has further been observed in the differentiating cementoblasts of the tooth periodontium at the site of tension after orthodontic tooth movement. Application of cyclic mechanical stress on PDL fibroblasts increases the expression of UNCL mRNA. These results indicate that UNCL plays important roles in the development, differentiation, and maintenance of periodontal tissues and also suggest a potential role of UNCL in the mechanotransduction of PDL fibroblasts.

Keywords

UNCL Periodontal ligament Fibroblast Mechanical stress Cementoblast Differentiation Human Rat (Sprague-Dawley)